文献类型：期刊文献

中文题名：硝酸银硅胶柱分离纯化蛇鲻鱼油中的EPA和DHA

英文题名：Separation and Purification of EPA and DHA from Saurida elongate Oil by Silver Nitrate-Silica Gel Column

作者：张良[1,2];刘美玲[1,2];董强[3];刘书成[1,2];郝记明[1,2];张静[1,2]

机构：[1]广东海洋大学食品科技学院,湛江524025;[2]广东省高等学校水产品深加工重点实验室,湛江524025;[3]西安市产品质量监督检验院,西安474100

年份：2011

卷号：26

期号：12

起止页码：63

中文期刊名：中国粮油学报

外文期刊名：Journal of the Chinese Cereals and Oils Association

收录：CSTPCD、、Scopus、CSCD2011_2012、北大核心2008、北大核心、CSCD

基金：2005粤港关键领域重点突破招标项目(2005A20301002);2007年广东省自然科学基金(7301127);广东海洋大学引进人才基金(0612044)

语种：中文

中文关键词：硝酸银-硅胶;DHA;EPA;分离

外文关键词：silver nitrate - silica gel, DHA, EPA, separation

中文摘要：采用NaOH-乙醇皂化再硫酸酸化的方法制备蛇鲻鱼油混合脂肪酸,然后用硝酸银硅胶柱色谱分离纯化EPA和DHA。结果表明:15 g硅胶与1 g硝酸银充分混合后装柱,置于5℃的恒温层析柜中,待色谱柱平衡后,上样0.75 g混合脂肪酸,然后依次用100 mL石油醚,2%,4%,8%,10%无水乙醚-石油醚溶液各100 mL进行洗脱,洗脱液流速为0.5～1.0 mL/min,收集200 mL以后的洗脱液,进行旋转蒸发,EPA和DHA的质量分数分别从4.43%和13.94%提高至21.47%及70.13%,总质量分数可达90%以上。

外文摘要：Free fatty acids from saurida elongate oil were prepared by saponification of NaOH - alcohol and a- cidification of H2SO4. EPA and DHA were then separated and purified by using silver nitrate - silica gel column. The results showed that 15 g silica gel and 1 g silver nitrate were mixed thoroughly and put into the constant temperature chromatography cabinet at 5 ℃. After the balance of the chromatographic column,0.75 g free fatty acid was added and eluted respectively by 100 mL petroleum ether, 100 mL petroleum ether of 2% diethyl ether, 100 mL petroleum ether of 4% diethyl ether, 100 mL petroleum ether of 8% diethyl ether, 100 mL petroleum ether of 10% diethyl ether. The eluting speed was controlled at 0.5 - 1.0 mL/min ; the elution was collected after 200 mL and evaporated by rotary evaporator. The contents of EPA and DHA were improved from 4.43 and 13.94% to 21.47% and 70.13% respectively. The total content of EPA and DHA was above 90%.