文献类型：期刊文献

英文题名：Effect of florfenicol on nirS-type denitrifying communities structure of water in an aquatic microcosm model

作者：Zhang, Tengyue[1];Peng, Jinju[1];Dai, Yue[1];Xie, Xingpeng[1];Luo, Shuaishuai[1];Ding, Yuexia[1];Ma, Yi[1,2]

机构：[1]Guangdong Ocean Univ, Coll Coastal Agr Sci, Dept Vet Med, Zhanjiang, Peoples R China;[2]Guangdong Lab Lingnan Modern Agr, Maoming Branch, Maoming, Peoples R China

年份：2023

卷号：10

外文期刊名：FRONTIERS IN VETERINARY SCIENCE

收录：SCI-EXPANDED(收录号：WOS:001039743200001)、、Scopus(收录号：2-s2.0-85166175897)、WOS

基金：This work was supported by the Natural Science Foundation of Guangdong Province, China (2023A1515012181); the Zhanjiang Science and Technology Bureau 2021 Provincial Science and Technology Special Funds (Big Special + Task List) Competitive Allocation Project (2021A05231); and the Start-Up Research Project of Maoming Laboratory, Guangdong Laboratory for Lingnan Modern Agriculture (2021TDQD002).

语种：英文

外文关键词：florfenicol; nirS gene; denitrifying microbial community; water body microorganisms; aquatic microcosm model

外文摘要：Florfenicol is used worldwide for its low side effects and strong bactericidal effect. Florfenicol is physicochemically stable and can persist in natural water bodies and affect water denitrification. Indoor aquatic microcosm models were constructed and water samples were collected at different florfenicol concentrations (0.1, 1, 10, and 100 mg/L) on days 0, 7, 30, and 60 to extract the microbial genome DNA and determine the water properties. qPCR and amplicon sequencing were used to study the dynamic changes of nirS gene and nirS-type denitrifying communities structure, diversity and abundance, respectively. The results showed that higher florfenicol concentrations caused accumulation of nitrate and ammonium nitrogen in water. Florfenicol stress caused orders of magnitude changes in nirS gene abundance, showing a trend of increasing first and then decreasing. 100 mg/L florfenicol addition led to a sustained increase of nirS gene abundance in water bodies. The florfenicol addition altered denitrifying community structure and suppressed the richness and diversity index of denitrifying bacteria in water body. Over time, the richness and diversity index gradually recovered. Proteobacteria was always the dominant denitrifying phylum in water. The relative abundance of Pseudomonas and beta proteobacterium showed obvious positive correlation with nirS gene abundance and were the dominant genera under florfenicol stress. Our study provided a scientific basis for the rational use of florfenicol in aquaculture to maintain a healthy and stable microecological environment, and also provided a preliminary understanding of the response characteristics of water denitrifying microorganisms to florfenicol exposure.