文献类型：期刊文献

英文题名：Detection of Aflatoxin B-1 Based on a Porous Anodized Aluminum Membrane Combined with Surface-Enhanced Raman Scattering Spectroscopy

作者：Feng, Yanting[1];He, Lei[2,3];Wang, Ling[1];Mo, Rijian[2,4];Zhou, Chunxia[1,2,4];Hong, Pengzhi[1,2,4];Li, Chengyong[2,3,4]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518108, Peoples R China;[3]Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China;[4]Southern Marine Sci & Engn Guangdong Lab, Zhanjiang 524088, Peoples R China

年份：2020

卷号：10

期号：5

外文期刊名：NANOMATERIALS

收录：SCI-EXPANDED(收录号：WOS:000540781800183)、、WOS

基金：This research is funded by the National Natural Science Foundation of China (21874029), Guangdong Yangfan Program (201635018), Guangdong Special Support Program (2017TQ04N706), Technology Planning Project of Zhanjiang City (2018A02014, 2016C01002), Science and Technology Planning Project of Shenzhen City (JCYJ20170818111719650), Industrial Development Special Funds of Dapeng New Area (KY20180202), Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang) (ZJW-2019-07, ZJW-2019-08), Innovation and Development Project about Marine Economy Demonstration of Zhanjiang City (2017C8B1) and the Graduate Education Innovation Planning Project of Guangdong Ocean University (201921).

语种：英文

外文关键词：surface-enhanced Raman scattering (SERS); Ag nanoparticles; porous anodized aluminum membrane; Aflatoxin B-1

外文摘要：An Aflatoxin B-1 (AFB(1)) biosensor was fabricated via an Ag nanoparticles assembly on the surface of a porous anodized aluminum (PAA) membrane. First, the Raman reporter 4-Aminothiophenol (4-ATP) and DNA (partially complementary to AFB(1) aptamer) were attached to the surface of Ag nanoparticles (AgNPs) by chemical bonding to form a 4-ATP-AgNPs-DNA complex. Similarly, the surface of a PAA membrane was functionalized with an AFB(1) aptamer. Then, the PAA surface was functionalized with 4-ATP-AgNPs-DNA through base complementary pairing to form AgNPs-PAA sensor with a strong Raman signal. When AFB(1) was added, AgNPs would be detached from the PAA surface because of the specific binding between AFB(1) and the aptamer, resulting in a reduction in Raman signals. The detection limit of the proposed biosensor is 0.009 ng/mL in actual walnut and the linear range is 0.01-10 ng/mL. The sensor has good selectivity and repeatability; it can be applied to the rapid qualitative and quantitative detection of AFB(1).