文献类型：期刊文献

英文题名：Transcriptional Analysis of Microcystis aeruginosa Co-Cultured with Algicidal Bacteria Brevibacillus laterosporus

作者：Zhang, Yulei[1,2];Chen, Dong[1];Zhang, Ning[1,2];Li, Feng[1,2];Luo, Xiaoxia[1];Li, Qianru[1];Li, Changling[1,2];Huang, Xianghu[1,2]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Dept Aquaculture, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Shenzhen Res Inst, Shenzhen 518108, Peoples R China

年份：2021

卷号：18

期号：16

外文期刊名：INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH

收录：SSCI(收录号：WOS:000689295600001)、SCI-EXPANDED(收录号：WOS:000689295600001)、、WOS

基金：This research was funded by the National Key R & D Plan "Blue Granary Science and Technology Innovation" (SQ2020YFD090053-05); the Guangdong Provincial Key Area Research and Development Plan Projects (2020B0202010009); the Guangdong South China Sea Key Laboratory of Aquaculture for Aquatic Economic Animals, Guangdong Ocean University (KFKT2019YB09); and the Natural Science Foundation of Guangdong Province (2018A030313212).

语种：英文

外文关键词：algicidal bacteria; Brevibacillus laterosporus; Microcystis aeruginosa; transcriptome; oxidative damage

外文摘要：Harmful algal blooms caused huge ecological damage and economic losses around the world. Controlling algal blooms by algicidal bacteria is expected to be an effective biological control method. The current study investigated the molecular mechanism of harmful cyanobacteria disrupted by algicidal bacteria. Microcystis aeruginosa was co-cultured with Brevibacillus laterosporus Bl-zj, and RNA-seq based transcriptomic analysis was performed compared to M. aeruginosa, which was cultivated separately. A total of 1706 differentially expressed genes were identified, which were mainly involved in carbohydrate metabolism, energy metabolism and amino acid metabolism. In the co-cultured group, the expression of genes mainly enriched in photosynthesis and oxidative phosphorylation were significantly inhibited. However, the expression of the genes related to fatty acid synthesis increased. In addition, the expression of the antioxidant enzymes, such as 2-Cys peroxiredoxin, was increased. These results suggested that B. laterosporus could block the electron transport by attacking the PSI system and complex I of M. aeruginosa, affecting the energy acquisition and causing oxidative damage. This further led to the lipid peroxidation of the microalgal cell membrane, resulting in algal death. The transcriptional analysis of algicidal bacteria in the interaction process can be combined to explain the algicidal mechanism in the future.