文献类型：期刊文献

中文题名：基于纳米碳酸钙的牡蛎肽递送体系构建与结构表征及其消化特性研究

英文题名：Construction,structural characterization and digestion characteristics of oyster peptides delivery system based on nano-calcium carbonate

作者：王婧[1,2];张萌萌[1,2];张卓铅[1,2];曹文红[1,2];谭明堂[1,2];朱国萍[1];高加龙[1];林海生[1];郑惠娜[1,2];陈忠琴[1,2]

机构：[1]广东海洋大学食品科技学院,国家贝类加工技术研发分中心(湛江),广东省水产品加工与安全重点实验室,广东省海洋生物制品工程实验室,广东省海洋食品工程技术研究中心,广东省水产预制食品加工与品质控制工程技术研究中心,广东湛江524088;[2]广东海洋大学深圳研究院,广东深圳518120

年份：2025

卷号：51

期号：19

起止页码：238

中文期刊名：食品与发酵工业

外文期刊名：Food and Fermentation Industries

收录：北大核心2023、、EI(收录号：20260520008410)、北大核心

基金：湛江市海洋青年人才创新项目(2021E05017);广东海洋大学科研启动费资助项目(060302042007);国家自然科学基金项目(32201971);广东省基础与应用基础研究基金项目(2024A1515011763);广东省现代农业产业技术体系贝藻创新团队项目(2024CXTD23)。

语种：中文

中文关键词：牡蛎肽;纳米碳酸钙;海藻酸钠修饰;纳米递送系统;消化特性

外文关键词：oyster peptides;nano-calcium carbonate;sodium alginate modification;nano delivery system;digestive stability

中文摘要：牡蛎肽具有抗氧化、降血糖、降血脂等多种生物活性,但其在胃肠道环境中稳定性差的问题严重限制了其活性发挥。为提高牡蛎肽的稳定性及减少牡蛎壳资源的浪费,该研究首先以牡蛎壳为原料制备了纳米碳酸钙(nano-calcium carbonate,CaCO_(3) NPs),然后采用海藻酸钠(sodium alginate,ALG)对其进行修饰,并优化修饰工艺;最后将牡蛎肽负载到ALG-CaCO_(3) NPs上,对其进行聚乙二醇修饰,构建了牡蛎肽-CaCO_(3) NPs纳米体系,并进一步探究其结构稳定性、体外释放及消化特性。正交试验结果表明,CaCO_(3) NPs的最佳修饰工艺参数为:ALG质量浓度5 mg/mL、温度35℃、反应时间2 h。结构表征结果表明,牡蛎肽-CaCO_(3) NPs纳米体系平均粒径为(288.7±7.6)nm,电位为(-30.5±0.8)mV,其包封率和装载量分别为(35.69±2.22)%和(19.71±1.60)%。傅里叶红外光谱表明ALG通过羧基与Ca^(2+)交联形成“蛋盒”结构对CaCO_(3) NPs进行修饰,而牡蛎肽是通过静电作用负载于纳米体系内部。体外释放实验结果显示,牡蛎肽-CaCO_(3) NPs纳米体系在模拟胃环境(pH 2.0)中36 h累积释放率仅为(40.31±0.63)%,而在肠环境(pH 6.8)中释放率显著提升至(93.12±3.76)%,表明其具有靶向肠道缓释特性。消化稳定性实验表明,牡蛎肽-CaCO_(3) NPs纳米体系可显著提高牡蛎肽的胰脂肪酶抑制活性,胃消化后抑制率从(23.66±0.93)%提升至(54.73±1.37)%,胃肠消化后抑制率从(4.75±0.38)%提升至(24.67±1.31)%。研究结果为功能性肽类的稳定化递送系统设计提供了理论支持,同时为牡蛎壳废弃物的高值化利用提供了新途径。

外文摘要：Oyster bioactive peptides(OBP)exhibit multiple biological activities including antioxidant,hypoglycemic,and hypolipidemic effects.However,the poor stability of OBP in gastrointestinal environments significantly limits their efficacy.To enhance the stability of OBP and reduce the waste of oyster shell resources,this study first synthesized nano-calcium carbonate(CaCO_(3) NPs)from oyster shell.The CaCO_(3) NPs were then modified with sodium alginate(ALG),and the modification process was optimized.Subsequently,OBP was loaded onto ALG-CaCO_(3) NPs,followed by polyethylene glycol modification,to construct an OBP-CaCO_(3) NPs nano-system.Finally,the study investigated the structural stability,in vitro release,and digestive characteristics of the nano-system.The orthogonal experimental results indicated that the optimal modification process parameters for CaCO_(3) NPs were:ALG concentration of 5 mg/mL,temperature of 35℃,and reaction time of 2 h.The nano-system showed an average particle size of(288.7±7.6)nm and zeta potential of(-30.5±0.8)mV,achieving encapsulation efficiency of(35.69±2.22)%and loading capacity of(19.71±1.60)%.Fourier transform infrared spectroscopy analysis revealed that ALG modified CaCO_(3) NPs by forming an‘egg box’structure through the cross-linking of carboxyl group and Ca^(2+),while OBP were loaded inside the carrier through electrostatic effect.The results of in vitro release experiments showed that the cumulative release rate of the nano-system in the simulated gastric environment(pH 2.0)for 36 hours was only(40.31±0.63)%,while the release rate in the intestinal environment(pH 6.8)significantly increased to(93.12±3.76)%,indicating the nano-system had the characteristics of targeted intestinal slow release.Digestion stability experiments demonstrated that the nano-system could significantly improve the pancreatic lipase inhibitory activity of OBP,the inhibitory rate increased from(23.66±0.93)%to(54.73±1.37)%after gastric digestion,and from(4.75±0.38)%to(24.67±1.31)%after gastrointestinal digestion.This study provides theoretical support for the design of stable delivery systems for functional peptides,while offering a novel approach for the high-value utilization of oyster shell waste.