文献类型：期刊文献

英文题名：Effects of Microplastics Exposure on the Acropora sp. Antioxidant, Immunization and Energy Metabolism Enzyme Activities

作者：Xiao, Baohua[1];Li, Dongdong[1];Liao, Baolin[1];Zheng, Huina[1];Yang, Xiaodong[1];Xie, Yongqi[1];Xie, Ziqiang[1];Li, Chengyong[1,2]

机构：[1]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen, Peoples R China;[2]Guangdong Ocean Univ, Sch Chem & Environm, Southern Marine Sci & Engn Guangdong Lab, Zhanjiang, Peoples R China

年份：2021

卷号：12

外文期刊名：FRONTIERS IN MICROBIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000662914900001)、、Scopus(收录号：2-s2.0-85108080272)、WOS

基金：The study was funded by the Guangdong Oceanic and Fishery Administration (project number: A201708D06), China Guangdong MEPP Fund [project number: GDOE (2019)A01], Shenzhen Science and Technology R&D Fund (project number: KJYY20180213182720347), Shenzhen Science and Technology R&D Fund (project number: JCYJ20200109144803833), Shenzhen Science and Technology R&D Fund (project number: KCXFZ202002011011057), Guangdong Key Area R&D Program Project (Project number:2020B1111030002), and China Guangdong OEDP Fund [project number: GDNRC (2020)040].

语种：英文

外文关键词：microplastics; Acropora sp; endosymbiont; enzyme; biochemical evaluation

外文摘要：Microplastic pollution in marine environments has increased rapidly in recent years, with negative influences on the health of marine organisms. Scleractinian coral, one of the most important species in the coral ecosystems, is highly sensitive to microplastic. However, whether microplastic causes physiological disruption of the coral, via oxidative stress, immunity, and energy metabolism, is unclear. In the present study, the physiological responses of the coral Acropora sp. were determined after exposure to polyethylene terephthalate (PET), polyamide 66 (PA66), and polyethylene (PE) microplastic for 96 h. The results showed that there were approximately 4-22 items/nubbin on the surface of the coral skeleton and 2-10 items/nubbin on the inside of the skeleton in the MPs exposure groups. The density of endosymbiont decreased (1.12 x 10(5)-1.24 x 10(5) cell/cm(2)) in MPs exposure groups compared with the control group. Meanwhile, the chlorophyll content was reduced (0.11-0.76 mu g/cm(2)) after MPs exposure. Further analysis revealed that the antioxidant enzymes in coral tissues were up-regulated (Total antioxidant capacity T-AOC 2.35 x 10(-3)-1.05 x 10(-2) mmol/mg prot, Total superoxide dismutase T-SOD 3.71-28.67 U/mg prot, glutathione GSH 10.21-10.51 U/mg prot). The alkaline phosphatase (AKP) was inhibited (1.44-4.29 U/mg prot), while nitric oxide (NO) increased (0.69-2.26 mu mol/g prot) for cell signal. Moreover, lactate dehydrogenase (LDH) was down-regulated in the whole experiment period (0.19-0.22 U/mg prot), and Glucose-6-phosphate dehydrogenase (G6PDH) for cell the phosphate pentoses pathway was also reduced (0.01-0.04 U/mg port). Results showed that the endosymbiont was released and chlorophyll was decreased. In addition, a disruption could occur under MPs exposure, which was related to anti-oxidant, immune, and energy metabolism.