文献类型：期刊文献

英文题名：Antimicrobial peptide AMPNT-6 from Bacillus subtilis inhibits biofilm formation by Shewanella putrefaciens and disrupts its preformed biofilms on both abiotic and shrimp shell surfaces

作者：Deng, Qi[1];Pu, Yuehua[2];Sun, Lijun[1];Wang, Yaling[1];Liu, Yang[3];Wang, Rundong[1];Liao, Jianmeng[4];Xu, Defeng[1];Liu, Ying[1];Ye, Riying[1];Fang, Zhijia[1];Gooneratne, Ravi[5]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Higher Educ Inst, Guangdong Prov Key Lab Aquat Prod Proc & Safety,K, 1 Haida Rd, Zhanjiang 524088, Guangdong, Peoples R China;[2]Bur Qual & Technol Supervise Zhanjiang City, Zhanjiang 524022, Peoples R China;[3]Natl Marine Prod Qual Supervis & Inspect Ctr, Zhanjiang 524000, Peoples R China;[4]Zhanjiang Food & Drug Adm, Zhanjiang 524000, Peoples R China;[5]Lincoln Univ, Dept Wine Food & Mol Biosci, Lincoln 7647, New Zealand

年份：2017

卷号：102

起止页码：8

外文期刊名：FOOD RESEARCH INTERNATIONAL

收录：SCI-EXPANDED(收录号：WOS:000419418100002)、、EI(收录号：20174004240530)、Scopus(收录号：2-s2.0-85030311722)、WOS

基金：The authors gratefully acknowledge financial support from the Public Research and Capacity Building Program of Guangdong Province (Nos. 2014B020204005 and 2014B020205006) and Guangdong Ocean University's higher education program for cultivating major scientific research projects (Nos. GDOU2013050205, 2014050203, 2013050312).

语种：英文

外文关键词：Bacillus subtilis; Antimicrobial lipopeptide; Shewanella putrefaciens; Anti-adhesion; Biofilm removal

外文摘要：Shewanella putrefaciens biofilm formation is of great concern for the shrimp industry because it adheres easily to food and food-contact surfaces and is a source of persistent and unseen contamination that causes shrimp spoilage and economic losses to the shrimp industry. Different concentrations of an antimicrobial lipopeptide, the fermentation product of Bacillus subtilis, AMPNT-6, were tested for the ability to reduce adhesion and disrupt S. putrefaciens preformed biofilms on two different contact surfaces (shrimp shell, stainless steel sheet). AMPNT-6 displayed a marked dose- and time-dependent, anti-adhesive effect > biofilm removal. 3MIC AMPNT-6 was able both to remove biofilm and prevent bacteria from forming biofilm in a 96-well polystyrene microplate used as the model surface. 2MIC AMPNT-6 prevented bacteria from adhering to the microplate surface to form biofilm for 3 h and removed already existing biofilm within 24 h. Secretion of extracellular polymeric substances incubated in LB broth for 24 h by S. putrefaciens was minimal at 3 x MIC AMPNT-6. Scanning electron microscopy showed that damage to S. putrefaciens bacteria by AMPNT-6 possibly contributed to the non-adherence to the surfaces. Disruption of the mature biofilm structure by AMPNT-6 contributed to biofilm removal. It is concluded that AMPNT-6 can be used effectively to prevent attachment and also detach S. putrefaciens biofilms from shrimp shells, stainless steel sheets and polystyrene surfaces.