文献类型：期刊文献

中文题名：转几丁质酶基因(RC24)水稻中大2号抗纹枯病特性研究

英文题名：Characters of Resistance to Rice Sheath Blight of Zhongda 2, a Transgenic Rice Line Modified by Chitinase Gene(RC24)

作者：袁红旭[1];许新萍[2];张建中[1];郭建夫[1];李宝健[2]

机构：[1]湛江海洋大学农学院,广东湛江524088;[2]中山大学生物工程研究中心,广东广州510275

年份：2004

卷号：18

期号：1

起止页码：39

中文期刊名：中国水稻科学

外文期刊名：Chinese Journal of Rice Science

收录：CSTPCD、、北大核心2000、Scopus、CSCD2011_2012、北大核心、CSCD

基金：国家 8 63计划资助项目 ( 10 10 1 0 2 0 2 );广东省教育厅自然科学基金资助项目 ( 2 0 0 0 40 );湛江海洋大学自然科学基金资助项目 ( 2 0 0 0 7)

语种：中文

中文关键词：转基因水稻;几丁质酶基因;纹枯病;抗病性

外文关键词：transgenic rice; chitinase gene; rice sheath blight; resistance

中文摘要：导入外源几丁质酶基因“RC2 4”的水稻中大 2号接种纹枯病菌的结果表明 ,接种病菌后病斑扩展速率和田间病情指数小 ,表现为对水稻纹枯病高抗。组织病理学研究表明纹枯病菌能侵入中大 2号 ,并引发纹枯病 ,入侵时间和症状出现的时间与非转基因感病对照没有显著差异 ,但病菌菌丝体消解现象出现早于对照 ,其抗病性主要表现为限制病菌扩展。以中大 2号与非转基因水稻材料配制的杂交组合的抗病性表明 ,杂交组合的抗病性均高于非转基因亲本 ,但其抗病性也会因母本的不同而有所变化。

外文摘要：The transgenic rice Zhongda 2, genetically modified from a indica rice cultivar Zhuxian B by rice chitinase gene (RC24), had high resistance to rice sheath blight (Rhizoctonia solani) in laboratory and a two-year field experiment. The pathogen could infect the sheath of Zhongda 2 and induce symptom of the disease. There was no difference in time of penetration or incubation period between Zhongda 2 and non-transgenic rice control, Zhuxian B, but the melting hypha was observed early compared with the control. Resistance of Zhongda 2 showed to inhibit the growth of mycelium in host tissue. The preliminary study on resistance of hybrid rice combinations (F 1) of Zhongda 2(♂) crossed withother five non-transgenic rice materials showed that the resistance of F 1generations were higher than those of non-transgenic maternal parents, but they also changed along with the different maternal parents.