文献类型：期刊文献

中文题名：雄烯二酮对赤点石斑鱼内分泌及性腺发育的影响

英文题名：Effects of ADSD implantation on endocrine and gonadal development in red-spotted grouper Epinephelus akaara

作者：舒琥[1,2];张勇[1];刘晓春[1];李广丽[1,3];林浩然[1,4]

机构：[1]中山大学有害生物控制与资源利用国家重点实验室,水生经济动物研究所和广东省水生经济动物良种繁育重点实验室;[2]广州大学生命科学学院,广州510405;[3]广东海洋大学水产学院,广东湛江524025;[4]海南大学海洋学院,海口570228

年份：2006

卷号：52

期号：2

起止页码：316

中文期刊名：动物学报

外文期刊名：ACTA ZOOLOGICA SINICA

收录：CSTPCD、、北大核心2004、Scopus、CSCD2011_2012、北大核心、CSCD、PubMed

基金：国家海洋863项目(No.2001AA621010;No.2003AA603011);国家自然科学基金项目(No.39970586);广东省科技计划项目(No.A3050201);(No.2003C20308);教育部科学技术研究重点项目(No.02150)资助~~

语种：中文

中文关键词：赤点石斑鱼;雄烯二酮;性逆转;性类固醇激素;芳香化酶表达和活性

外文关键词：Epinephelus akaara, ADSD, Sex reversal, Sex steroid hormone, Aromatase expression and activity

中文摘要：采用腹部埋植法,应用连续组织切片技术、放射免疫测定方法和半定量RT-PCR技术,检测了间隔30d3次埋植不同剂量(高剂量H:100μg/g体重,低剂量L:10μg/g体重)的雄烯二酮(4-androstene-3,17-dine,ADSD)诱导2龄赤点石斑鱼性腺发育过程中血清性类固醇激素含量、芳香化酶表达和活性的变化。结果显示:第1次埋植ADSD后30d即可诱导雌性赤点石斑鱼发生不同程度的性逆转,性腺成熟指数明显下降,性腺中卵细胞退化,精原细胞增殖,出现大量精母细胞和精子细胞,第1次埋植ADSD后30d经轻微挤压腹部有10%(低剂量组)和15%(高剂量组)的鱼可排精;第2次埋植后30d明显提高性逆转效果,排精率分别达到80%和86·7%,性逆转雄鱼的精巢在组织结构上与正常雄鱼精巢一样,精子活力与正常雄鱼相同,对照组鱼卵巢发育良好;第3次埋植后30d排精率分别为90%和80%,埋植ADSD后性腺芳香化酶(P450aromA)表达和活性显著降低,脑芳香化酶(P450aromB)表达显著升高,前脑芳香化酶活性显著升高,血清T,11-KT水平显著高于对照组,而血清E2水平先升高,然后逐渐降低。说明血清T,11-KT水平增加,性腺芳香化酶表达及活性降低在控制雌雄同体的赤点石斑鱼的性转化和精子发生中起重要作用[动物学报52(2):316-327,2006]。

外文摘要：Through the detection methods of histology, radioimmunoassay （RIA） and RT-PCR, the effects of various dose of ADSD via implantation on sex steroid contents, aromatase expression and activity in 2-year-old red-spotted groupe Epinephelus akaara were examined. Groupers were divided into three groups, one implanted with high 4-androstene-3, 17-dine （ADSD, 100 μzg/g body weight ）, the second with low ADSD （ 10 μg/g body weight） and the third without ADSD once 30d for 3 months. The results indicated sex inversion in ADSD-implanted group. Fish implanted with ADSD once （for 30 d） showed significantly lower GSI and complete degradation of oocytes and developing testicular tissues. ADSD induced females to develop into functionalimales, these males had testes, which were similar in structure from those of normal males. Spermiating rates of induced males in ADSD-implanted group as follows： 15%, 86.7% and 80% （high dose group）, or 10% , 80% and 90% （low dose group） at the 30 d, 60 d, and 90d, respectively, and the sperm motility was the same between the control and ADSD-implantation, while all fish in the control were still female with developing ovaries. Aromatase expression and activity decreased significantly in the gonad, increased significantly in forebrain, respectively, after ADSD implanted. Serum estradiol-17β （E2） levels began to increase after ADSD-implantation, then decreased gradually in ADSD-treated group, while serum levels of testosterone （T） and 11-ketotestosterone （11-KT） concentration increased significantly. The results suggest that the increase of serum T, 11-KT levels and gonadal aromatase expression and activity may be important to the controlled male phase in protogynous red-spotted grouper, E. akaara .