文献类型：期刊文献

英文题名：The isolation, identification, whole-genome sequencing of Clostridium butyricum LV1 and its effects on growth performance, immune response, and disease-resistance of Litopenaeus vannamei

作者：Wang, Qi[1,2,3,5]; Li, Weikang[3,4]; Liu, Hongyu[1,2,3]; Tan, Beiping[1,2,3]; Dong, Xiaohui[1,2,3]; Chi, Shuyan[1,2,3]; Yang, Qihui[1,2,3]; Zhang, Shuang[1,2,3]; Fan, Depeng[5]; Hu, Yadong[5]

机构：[1] Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang, 524025, China; [2] Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Centre of Guangdong Province, Guangdong, Zhanjiang, China; [3] Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, 524025, China; [4] Guangdong Evergreen Feed Industry Co., Ltd, Zhanjiang, 524088, China; [5] Bio-Form Biotechnology [Guangdong] Co., Ltd, Foshan, 528200, China

年份：2023

卷号：272

外文期刊名：Microbiological Research

收录：EI(收录号：20231814038694)、Scopus(收录号：2-s2.0-85153948858)

语种：英文

外文关键词：Antibiotics - Clostridium - Hydrolases - Immune system - Phosphatases - Probiotics

外文摘要：In this study, a strain of Clostridium butyricum was isolated from the intestine of Litopenaeus vannamei with the method of anaerobic microbial isolation and culture. Next, the probiotic properties of LV1 were evaluated with susceptibility tests, tolerance tests, and whole genome sequencing in vivo and in vitro, followed by the analysis of the effect of LV1 on the growth performance, immune response, and disease resistance of Litopenaeus vannamei. According to the results, the 16 S rDNA sequence of LV1 was 100% homolofgous to the reference sequence of Clostridium butyricum. Moreover, LV1 was resistant to several antibiotics including amikacin, streptomycin, and gentamicin and highly tolerated artificial gastric and artificial intestinal fluids. The whole genome of LV1 was 4625,068 bp in size and included 4336 coding genes. Among these genes, GO, KEGG, and COG databases exhibited the highest number of genes annotated to metabolic pathway classes and 105 genes annotated as glycoside hydrolases. Meanwhile, 176 virulence genes were predicted. The use of diets supplemented with 1.2 × 109 CFU/kg of LV1 live cells significantly increased the weight gain and specific growth rates of Litopenaeus vannamei and the activity of serum superoxide dismutase, glutathione peroxidase, acid phosphatase, and alkaline phosphatase (P 9 CFU/kg of LV1 live cells to the diet improved the growth performance, immune response, and disease-resistance of Litopenaeus vannamei. ? 2023 Elsevier GmbH