文献类型：期刊文献

中文题名：Single Nucleotide Polymorphism (SNP) Analysis of orexin Gene 5' Regulatory Region in Chinese Indigenous Cattle Populations

英文题名：Single Nucleotide Polymorphism (SNP) Analysis of orexin Gene 5' Regulatory Region in Chinese Indigenous Cattle Populations

作者：ZHANG Ai-ling[1,2];ZHANG Li[1,3];ZHANG Liang-zhi[1];LAN Xian-yong[1];ZHANG Cun-lei[4];ZHANG Cun-fang[1];CHEN Hong[1,4]

机构：[1]Shaanxi Key Laboratory of Molecular Biology for Agriculture,College of Animal Science and Technology,Northwest A＆F University,Yangling 712100,P.R.China;[2]Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding,College of Animal Science,South China Agricultural University,Guangzhou 510642,P.R.China;[3]Agricultural College,Guangdong Ocean University,Zhanjiang 524000,P.R.China;[4]College of Life Sciences,Xuzhou Normal University,Xuzhou 221000,P.R.China

年份：2011

卷号：10

期号：8

起止页码：1273

中文期刊名：Agricultural Sciences in China

外文期刊名：中国农业科学（英文版）

收录：Scopus、CSCD2011_2012、CSCD

基金：This study was supported by the National Natural Science Foundation of China (30972080), the National Key Technology R&D Program of China (2008BADB2B03-19), the Keystone Project of Transfergene in China (2009ZX08009-157B, 2008ZX08007-002, 2009ZX080 07-005B-07), the Program of National Beef Cattle Industrial Technology System, China (CARS-38), China Postdoctoral Science Foundation Founded Project (20100480763), and the Doctor Seed Grant of Guangdong Ocean University, China (0712130).

语种：英文

中文关键词：orexin gene; promoter; SNP; cattle

外文关键词：orexin gene; promoter; SNP; cattle

中文摘要：Orexin is an important neuropeptide that influences livestock＇s appetite and food intake and is closely related with livestock＇s growth and development.The variations in the orexin gene 5＇ regulatory region might have an influence on the gene expression.Based on the hypothesis,five overlapped fragments including 1 794 bp of orexin gene 5＇ regulatory region were investigated for single nucleotide polymorphisms （SNPs） by PCR-SSCP and DNA sequencing in three indigenous cattle populations.A total of five SSCP patterns observed revealed ten SNPs in the region.Two SSCP patterns genotypes （A and B） were exhibited in O-2 fragment and three （A,B and C） were found in O-5 fragment.O-2 contained four SNPs,viz.,-583 TC,-479 CT,-474 AT,and-451 AG.In another lous O-5,six SNPs were identified （-1 610 CG,-1 585 GA,-1 550 TC,-1 548 AC,-1 438 CT,and-1 431 CA）.Seven SNPs were found in transcription factor binding sites and four out of them existed in the core sequences.The SNPs at-479,-474 and-451 did not change the putative recognition core sequences of their factors.But the mutation at-583 changed the binding sequence of EVI1 into NFA and created one new binding site for ZFHX simultaneously.In three populations,the frequencies of A,B and C genotypes of O-2 were 0.2367,0.4842 and 0.2791,respectively.And the A pattern of O-5 was preponderant （0.7549） and the other B pattern was not （0.2451）.But the frequencies of different SSCP variants varied across three cattle populations.B genotype in O-2 had significant associations to body weight （BW） and daily weight gain （DWG） in Nanyang cattle 6-and 12-mon aged and might serve as one potential candidate genetic marker for growth and development.

外文摘要：Orexin is an important neuropeptide that influences livestock＇s appetite and food intake and is closely related with livestock＇s growth and development.The variations in the orexin gene 5＇ regulatory region might have an influence on the gene expression.Based on the hypothesis,five overlapped fragments including 1 794 bp of orexin gene 5＇ regulatory region were investigated for single nucleotide polymorphisms （SNPs） by PCR-SSCP and DNA sequencing in three indigenous cattle populations.A total of five SSCP patterns observed revealed ten SNPs in the region.Two SSCP patterns genotypes （A and B） were exhibited in O-2 fragment and three （A,B and C） were found in O-5 fragment.O-2 contained four SNPs,viz.,-583 TC,-479 CT,-474 AT,and-451 AG.In another lous O-5,six SNPs were identified （-1 610 CG,-1 585 GA,-1 550 TC,-1 548 AC,-1 438 CT,and-1 431 CA）.Seven SNPs were found in transcription factor binding sites and four out of them existed in the core sequences.The SNPs at-479,-474 and-451 did not change the putative recognition core sequences of their factors.But the mutation at-583 changed the binding sequence of EVI1 into NFA and created one new binding site for ZFHX simultaneously.In three populations,the frequencies of A,B and C genotypes of O-2 were 0.2367,0.4842 and 0.2791,respectively.And the A pattern of O-5 was preponderant （0.7549） and the other B pattern was not （0.2451）.But the frequencies of different SSCP variants varied across three cattle populations.B genotype in O-2 had significant associations to body weight （BW） and daily weight gain （DWG） in Nanyang cattle 6-and 12-mon aged and might serve as one potential candidate genetic marker for growth and development.