文献类型：期刊文献

英文题名：Rapid surface enhanced Raman scattering detection method for chloramphenicol residues

作者：Ji, Wei[1];Yao, Weirong[2]

机构：[1]Guangdong Ocean Univ, Sch Food Sci & Technol, Zhanjiang City 524088, Guangdong, Peoples R China;[2]Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

年份：2015

卷号：144

起止页码：125

外文期刊名：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

收录：SCI-EXPANDED(收录号：WOS:000352668100016)、、EI(收录号：20151000613683)、Scopus(收录号：2-s2.0-84924075369)、WOS

基金：This article is supported by the National Natural Science Foundation of People Republic of China (No. 21203076), National Key Technology R&D Program in the 12th Five year Plan of China (No. 2012BAD36B02), Key Technology R&D Program in Jiangsu province (No. BE2012631), Open Project of State Key Laboratory of Supramolecular Structure and Materials (sklssm 201328), the Fundamental Research Funds for the Central Universities (JUSRP51309A) and the Priority Academic Program Development of Jiangsu Higher Education Institution (PAPD).

语种：英文

外文关键词：Chloramphenicol (CAP); Gold colloidal nanoparticles; Surface enhanced Raman scattering (SERS); Quantitative analysis; Density functional theory (DFT)

外文摘要：Chloramphenicol (CAP) is a widely used amide alcohol antibiotics, which has been banned from using in food producing animals in many countries. In this study, surface enhanced Raman scattering (SEAS) coupled with gold colloidal nanoparticles was used for the rapid analysis of CAP. Density functional theory (DFT) calculations were conducted with Gaussian 03 at the B3LYP level using the 3-21G(d) and 6-31G(d) basis sets to analyze the assignment of vibrations. Affirmatively, the theoretical Raman spectrum of CAP was in complete agreement with the experimental spectrum. They both exhibited three strong peaks characteristic of CAP at 1104 cm(-1), 1344 cm(-1), 1596 cm(-1), which were used for rapid qualitative analysis of CAP residues in food samples. The use of SERS as a method for the measurements of CAP was explored by comparing use of different solvents, gold colloidal nanoparticles concentration and absorption time. The method of the detection limit was determined as 0.1 mu g/mL using optimum conditions. The Raman peak at 1344 cm(-1) was used as the index for quantitative analysis of CAP in food samples, with a linear correlation of R-2 = 0.9802. Quantitative analysis of CAP residues in foods revealed that the SERS technique with gold colloidal nanoparticles was sensitive and of a good stability and linear correlation, and suited for rapid analysis of CAP residue in a variety of food samples. (C) 2015 Elsevier B.V. All rights reserved.