文献类型：期刊文献

中文题名：鳕鱼皮多肽抗小鼠慢性酒精性肝、脑损伤作用

英文题名：Protective Effects of Cod Skin Collagen Peptides against Chronic Alcoholic Liver and Brain Injury in Mice

作者：陈晨[1];郭家棋[1];孔松芝[1];谢海生[1];梁美茵[1];陈锦蕙[1];利紫芮[1]

机构：[1]广东海洋大学化学与环境学院,广东湛江524088

年份：2025

卷号：46

期号：11

起止页码：225

中文期刊名：食品科学

外文期刊名：Food Science

收录：北大核心2023、、EI(收录号：20252218492801)、北大核心

基金：2023年度广东省普通高校重点科研平台和项目——广东省普通高校重点领域专项(生物医药与健康)(2023ZDZX2027)。

语种：中文

中文关键词：鳕鱼皮多肽;酒精性肝损伤;酒精性脑损伤;慢性酒精中毒

外文关键词：codfish skin polypeptides;alcoholic liver injury;alcoholic brain injury;chronic alcoholism

中文摘要：采用长期灌胃乙醇溶液法建立小鼠慢性酒精性损伤模型,通过对比各组小鼠肝、脑组织中的生化指标水平及其病理组织学检查结果,探讨鳕鱼皮多肽改善慢性酒精性肝、脑损伤的作用及其可能的作用机制。研究结果显示,与模型组相比,鳕鱼皮多肽处理能在一定程度上降低小鼠血清中甘油三酯的含量以及谷草转氨酶和碱性磷酸酶的活性,提高肝脏组织中乙醇脱氢酶、乙醛脱氢酶、谷胱甘肽过氧化物酶及过氧化氢酶的活性,有效抑制肝脏组织中肿瘤坏死因子α、白细胞介素-8等炎症因子的高表达,降低细胞色素P450的含量;同时提高小鼠脑组织中乙酰胆碱转移酶、脑源性神经营养因子的含量,有效抑制其脑组织中肿瘤坏死因子α、白细胞介素-1β、白细胞介素-6等关键炎症因子以及烟酰胺磷酸核糖转移酶的高表达,抑制诱导型一氧化氮合酶和一氧化氮的合成,提高谷胱甘肽过氧化物酶、超氧化物歧化酶及过氧化氢酶的活性,降低胆碱酯酶、B型单胺氧化酶、丙二醛、活性氧的水平。此外,病理组织学检查显示,相对于模型组小鼠,鳕鱼皮多肽处理后的小鼠肝脏结构清晰,肝小叶排列整齐,少见水样和肝细胞脂肪变性,胶原纤维的生成少。上述结果表明,鳕鱼皮多肽可在一定程度上通过调控核因子κB信号通路、核因子κB信号通路/一氧化氮合酶-一氧化氮信号通路分别缓解乙醇诱导的肝、脑氧化应激和炎性反应,同时还能通过增强肝脏乙醇代谢酶活性促进乙醇的代谢以及正向调节脑内神经中枢系统水平以维持神经递质系统的平衡,进而有效缓解肝、脑损伤。

外文摘要：In this study,a mouse model of chronic alcohol-induced liver and brain injury was established through prolonged intragastric administration of an alcohol solution.By comparing biochemical parameters and histopathological findings in liver and brain tissues across different experimental groups,we investigated the effects of codfish skin polypeptides on chronic alcohol-induced organ damage and the underlying mechanisms.The results demonstrated that,compared with the model group,treatment with codfish skin polypeptides significantly reduced serum triglyceride(TG)levels and the activities of aspartate aminotransferase(AST)and alkaline phosphatase(ALP).It also enhanced the activities of alcohol dehydrogenase(ADH),aldehyde dehydrogenase(ALDH),glutathione peroxidase(GSH-Px),and catalase(CAT)in liver tissue,and effectively inhibited the overexpression of inflammatory cytokines such as tumor necrosis factor-α(TNF-α)and interleukin-8(IL-8),while reducing cytochrome P450(CYP450)content.In brain tissue,codfish skin polypeptides increased the levels of acetylcholine transferase(ChAT)and brain-derived neurotrophic factor(BDNF),suppressed the overexpression of key inflammatory factors including TNF-α,interleukin-1β(IL-1β),interleukin-6(IL-6),and nicotinamide phosphoribosyltransferase(NAMPT),inhibited inducible nitric oxide synthase(iNOS)and nitric oxide(NO)synthesis,elevated glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),and catalase(CAT)activities,and decreased cholinesterase(AChE),monoamine oxidase B(MAO-B),malondialdehyde(MDA),and reactive oxygen species(ROS)levels.Histopathological examination revealed that,compared with the model group,the liver structure of codfish skin polypeptides-treated mice was clearer with better organized hepatic lobules,less hydropic and fatty degeneration of hepatocytes,and reduced collagen fiber deposition.These findings suggest that codfish skin polypeptides alleviate alcoholinduced oxidative stress and inflammatory responses in both liver and brain tissues by modulating the NF-κB signaling pathway and the NF-κB/iNOS-NO signaling pathway,respectively.Additionally,these polypeptides promote ethanol metabolism by enhancing the activity of hepatic ethanol-metabolizing enzymes and positively regulate the central nervous system to maintain neurotransmitter balance,thereby effectively mitigating alcohol-induced liver and brain injury.