文献类型：期刊文献

中文题名：火龙果皮原花青素提取纯化及定性分析

英文题名：Extraction, Purification and Qualitative Analysis of Proanthocyanidins from Pitaya Peel

作者：杨志娟[1];曾真[1];吴晓萍[1]

机构：[1]广东海洋大学食品科技学院

年份：2015

卷号：0

期号：2

起止页码：75

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD、、北大核心2014、Scopus、北大核心、CSCD_E2015_2016、CSCD

语种：中文

中文关键词：火龙果皮;原花青素;提取纯化;定性分析

外文关键词：pitaya peel;proanthocyanidins;extraction and purification;qualitative analysis

中文摘要：采用大孔树脂对原花青素粗品进行分离纯化。比较了AB-8、DM130、ADS-17三种大孔树脂对原花青素的吸附和解吸能力,选择AB-8型大孔树脂为吸附树脂。对AB-8大孔树脂吸附解吸条件进行优化,探讨上样流速、解吸流速和解吸剂体积分数3个因素对分离纯化效果的影响。结果表明:AB-8大孔树脂提纯工艺的最佳条件为上样流速2 m L/min、解吸流速1 m L/min、解吸剂体积分数50%。通过花青素反应与高效液相色谱-质谱对提取物进行定性分析,证实该提取物中含有的原花青素为儿茶素、表儿茶素和二聚体,且原花青素纯度为96.65%。

外文摘要：The crude proanthocyanidins extracted from pitaya peel were purified by macroporous adsorption resin. AB-8 macroporous resin was selected as the best adsorbent for proanthocyanidins based on adsorption and desorption capacities in comparison with DM130 and ADS-17. Effects of sample loading flow rate, elution flow rate and eluent concentration on the purification of pitaya peel proanthocyanidins by AB-8 macroporous resin were investigated. The best results were achieved by sample loading at a flow rate of 2 m L/min followed by elution with 50% ethanol at a flow rate of 1 m L/min. The purified product contained 96.65% proanthocyanidins including catechin, epicatechin and dimmer as indicated by colorimetric observation and qualitative analysis by high performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-ESI-MS).