文献类型：期刊文献

英文题名：New function of the CD44 gene: Lipid metabolism regulation in bovine mammary epithelial cells

作者：Jiang, Ping[1];Xia, Lixin[2];Jin, ZiKang[1];Ali, Shaokat[1];Wang, Mengyan[2];Li, Xiaohui[2];Yang, Runjun[2];Fang, Xibi[2];Zhao, Zhihui[1]

机构：[1]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524088, Peoples R China;[2]Jilin Univ, Coll Anim Sci, Changchun 130062, Peoples R China

年份：2020

卷号：103

期号：7

起止页码：6661

外文期刊名：JOURNAL OF DAIRY SCIENCE

收录：SCI-EXPANDED(收录号：WOS:000541147200002)、、Scopus(收录号：2-s2.0-85084063270)、WOS

基金：This work was supported by the National R&D Project of Transgenic Organisms of the Ministry of Science and Technology of China (2016ZX08009003-006; Beijing), the National Natural Science Foundation of China (No. 31772562; Beijing), the Key Platform Project of Innovative Strong School by Education Department of Guangdong Province (ZhanJiang, 2018302), and the Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction and the Ministry of Agriculture (Poultrylab2018-4; Beijing, China). The authors declare no competing financial interests.

语种：英文

外文关键词：bovine mammary epithelial cells; CD44; cholesterol; fatty acids; triglycerides

外文摘要：The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis. We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents. Our results suggest that CD44 might be a candidate gene affecting milk fat synthesis. In the present study, the overexpression of the CD44 gene increased the contents of intracellular triglycerides (TG) and cholesterol (CHOL), whereas knockdown of the CD44 gene decreased bMEC CHOL and TG contents. Gas chromatography analysis of fatty acid composition showed that the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid were altered due to changes in the level of expression of the CD44 gene. Additionally, elaidic acid, palmitoleic acid, tridecanoic acid, and oleic acid were markedly reduced in the CD44 gene overexpression group compared with the control group. On the contrary, cis-5,8,11,14-eicosatetraenoic acid and stearic acid were markedly increased in the CD44 knockdown group compared with the control group. And RT2 Pro?ler PCR array (Qiagen, CLAB24070A Frankfurt, Germany) further suggested that overexpression or knockdown of the CD44 gene altered expression levels of functional genes associated with lipid metabolism. The present data indicate that CD44 plays a key regulatory role in lipid metabolism in bMEC. ? 2020 American Dairy Science Association