文献类型：期刊文献

英文题名：Integration of Histology, Transcriptome and Metabolome Reveals the Effect of Cold Stress on Cobia (Rachycentron Canadum)

作者：Zhao, Xiaolong[1]; Li, Yu[1]; Ma, Qian[1]; Huang, Jiansheng[1]; Zhang, Jiandong[1]; Chen, Gang[1]

机构：[1] College of Fisheries, Guangdong Ocean University, Zhanjiang, 524088, China

年份：2024

外文期刊名：SSRN

收录：EI(收录号：20240454340)

语种：英文

外文关键词：Amino acids - Amino sugars - Gene expression - Gene expression regulation - Liver

外文摘要：Cobia (Rachycentron canadum) is ideal for marine cage culture in the South China Sea. To investigate its response to cold stress, an integration of histological, transcriptomic, and metabolomic analysis of the liver was conducted. Fish were subjected to cold stress at 18 °C, and liver samples were collected at three sampling time points under cold stress (0, 4, and 7 D). Histological examination of the liver revealed cytoplasm vacuolization of the hepatocytes and accumulation of lipid droplets under cold stress. In addition, the microRNA (miRNA) and mRNA expression profiles in the liver were examined using the Illumina Novaseq 6000 platform. As a result, a total of 90 differentially expressed (DE) miRNAs and 86 predicted target genes were identified, among which 6 miRNAs and 43 target genes were significantly clustered in significant changing trends. KEGG analysis of these genes showed that they were significantly enriched in galactose metabolism and amino sugar and nucleotide sugar metabolism pathways. The weighted gene co-expression network analysis (WGCNA) of the DE genes (DEGs) has obtained three modules that was significantly correlated with cold stress, and KEGG analysis of the genes in these modules were significantly enriched in 8 metabolism-related pathways (such as Steroid biosynthesis, Glutathione metabolism, etc.) and 20 other terms. Furthermore, a total of 280 significantly regulated metabolites (SRMs) were identified using an LC-ESI-MS/MS system. An integrative transcriptome and metabolome analysis suggested that the DEGs and SRMs were significantly enriched in glycerophospholipid metabolism and biosynthesis of amino acids pathways. The genes and metabolites identified in this study might play an important role in the regulation of the cold stress response of cobia, and the investigation could provide new insights into the molecular mechanisms underlying the adaptation of teleost species to climate change. ? 2024, The Authors. All rights reserved.