文献类型：期刊文献

中文题名：低氧胁迫对军曹鱼幼鱼抗氧化、免疫能力及能量代谢的影响

英文题名：Effects of Hypoxia Stress on Antioxidation,Immunity and Energy Metabolism of Juvenile Cobia,Rachycentron canadum

作者：王维政[1];曾泽乾[1];黄建盛[1,2];郭志雄[1];李洪娟[1];陈刚[1,2]

机构：[1]广东海洋大学水产学院,广东湛江524088;[2]南方海洋科学与工程广东省实验室(湛江),广东湛江524025

年份：2020

卷号：40

期号：5

起止页码：12

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：CSTPCD

基金：现代农业产业技术体系专项资金资助(CARS-47);广东海洋大学大学生创新创业训练计划项目(CXXL2018131);南方海洋科学与工程广东省实验室(湛江)(ZJW-2019-06);2018年广东海洋大学起航计划项目(QHJHZR201818)。

语种：中文

中文关键词：军曹鱼;低氧胁迫;抗氧化;免疫;能量代谢

外文关键词：Rachycentron canadum;hypoxia;antioxidant;immune;energy metabolism

中文摘要：【目的】研究低氧胁迫对军曹鱼(Rachycentron canadum)幼鱼抗氧化、免疫能力和能量代谢的影响。【方法】幼鱼于(2.98±0.40) mg/L的低溶氧条件下养殖1周,分别测定其肝脏和肌肉组织的抗氧化、免疫相关酶活力以及能量供应物质。【结果】低氧胁迫过程中,幼鱼肝脏组织超氧化物歧化酶(SOD)活力显著升高(P <0.05)后逐渐下降(P <0.05),肌肉组织SOD活力呈波动上升趋势(P <0.05),肝脏和肌肉组织谷胱甘肽过氧化物酶(GPX)活力和丙二醛(MDA)含量均显著升高(P<0.05)后,呈逐渐下降趋势,肝脏组织过氧化氢酶(CAT)先降(P<0.05)后升至正常水平,肌肉组织CAT活力先升(P <0.05)后呈下降趋势;肝脏组织碱性磷酸酶(AKP)活力先降后升高(P <0.05),随后恢复至与对照组水平(P> 0.05),肝脏酸性磷酸酶(ACP)活力先显著上升(P <0.05),随后恢复至正常水平(P> 0.05);肌肉组织和肝脏组织乳酸脱氢酶(LDH)活力变化趋势一致,均为先显著升高(P<0.05),之后呈降低趋势;肝糖原在低氧胁迫后呈先下降(P <0.05),后恢复至对照组水平(P> 0.05),肌糖原各时间点含量无显著性差异(P> 0.05)。【结论】军曹鱼幼鱼在低氧胁迫后发生氧化损伤,刺激自身免疫系统,通过调整相关酶活力及能量代谢的方式提高其适应低氧的能力。

外文摘要：【Objective】To investigate the effect of hypoxia on antioxidant,immunity and energy metabolism of juvenile cobia.【Method】Rachycentron canadum,a 7-day hypoxia(2.98±0.40 mg/L)experiment was conducted,and the enzymes of antioxidant,immunity and the energy metabolism in liver and muscle were monitored.【Result】The activity of superoxide dismutase(SOD)increased significantly(P<0.05)and then decreased in liver while significantly increased(P<0.05)in muscle after hypoxia stress.The activities of glutathione peroxidase(GPX)and the content of malondialdehyde(MDA)in both liver and muscle increased significantly(P<0.05)and then decreased.However,the activities of catalase(CAT)increased significantly(P<0.05)and then decreased in muscle,whereas it is decreased significantly(P<0.05)and then increased in liver.Alkaline phosphatase(AKP)activity in liver decreased significantly(P<0.05)at first and then increased significantly(P<0.05),and eventually recovered to the level of control,but the activities of acid phosphatase(ACP)presented the opposite trend.The activities of lactate dehydrogenase(LDH)was the same in liver and muscle,both increased significantly(P<0.05)and then decreased.In addition,the content of glycogen in liver declined(P<0.05)after hypoxia stress,then recovered to the level of the control(P>0.05).but the content of muscle glycogen had no significant difference(P>0.05)in different time.【Conclusion】The juvenile cobia encountered oxidative damage after hypoxia stress,which stimulated the immune system and improved its ability to adapt to hypoxia by regulating the activity of relevant enzymes and energy metabolism.