文献类型：期刊文献

英文题名：Enhanced flavonoid production in hairy root cultures of Glycyrrhiza uralensis Fisch by combining the over-expression of chalcone isomerase gene with the elicitation treatment

作者：Zhang, Hai-Chao[1];Liu, Jing-Mei[2];Lu, Hong-Yu[3];Gao, Shan-Lin[1]

机构：[1]China Pharmaceut Univ, Dept Breeding & Genet, Nanjing 210038, Jiangsu Prov, Peoples R China;[2]Natl Ctr Mol Crop Design, Beijing 100085, Peoples R China;[3]Guangdong Ocean Univ, Food Sci & Technol Coll, Zhanjiang 524025, Guangdong, Peoples R China

年份：2009

卷号：28

期号：8

起止页码：1205

外文期刊名：PLANT CELL REPORTS

收录：SCI-EXPANDED(收录号：WOS:000268500400006)、、Scopus(收录号：2-s2.0-69449097014)、WOS

基金：This research was supported by grants from National Centre for Molecular Crop Design and from the National '863' Progra (No. 2007AA021504) in Peking University, China. The authors are very grateful to Professor Xi-Ping Wang and assistants Ying Xie and Hai-Min Chen of National Centre for Molecular Crop Design for their advices and help.

语种：英文

外文关键词：Chalcone isomerase(chi); Elicitor; Flavonoids; Genetic transformation; Glycyrrhiza uralensis Fisch; Hairy roots

外文摘要：Economically important compounds, such as licorice flavonoids, are present in insufficient amounts in the hairy roots. To overcome this problem, we took the transgenic approach combined with the elicitation technique to increase the flavonoid production. The Glycyrrhiza uralensis Fisch cDNA encoding chalcone isomerase gene (chi) was over-expressed in hairy roots of G. uralensis Fisch mediated by the disarmed Agrobacterium rhizogenes A4. Stable genetic transformation was confirmed by Southern blot analysis. The transgenic and wild cultures were subsequently elicited with PEG8000 (2%) alone, yeast extract (YE) (0.1%) alone, or both of them, and then the total flavonoids were extracted and measured. The results showed that over a culture period of 3 weeks, the wild-type hairy roots, the untreated transgenic hairy roots, and the double-treated transgenic hairy roots accumulated 0.842, 1.394, and 2.838 (g/100 g DW) of total flavonoids, respectively. Moreover, the enhanced accumulation of flavonoids were correlated with the elevated level of chi transcripts and CHI activity, confirming the key role of chi in the flavonoids synthesis. This research demonstrated that the combination of the metabolic engineering and PEG8000-YE elicitation treatment was an effective strategy to increase the flavonoids production in hairy roots of G. uralensis Fisch.