文献类型：期刊文献

中文题名：龙趸石斑鱼肝脏维生素A的形态分布及其对干眼症细胞模型的影响

英文题名：Speciation Distribution of Vitamin A from Liver of Epinephelus drummondhayi and Its Effect on Xerophthalmia Cell Model

作者：张静[1,2];黄文婷[1];曹文红[1,2];陈忠琴[1,2];郑惠娜[1,2];高加龙[1,2];章超桦[1,2]

机构：[1]广东海洋大学食品科技学院/国家贝类加工技术研发分中心(湛江)/广东省水产品加工与安全重点实验室/广东省海洋食品工程技术研究中心/广东省海洋生物制品工程实验室/水产品深加工广东普通高等学校重点实验室,广东湛江524088;[2]大连工业大学海洋食品精深加工关键技术省部共建协同创新中心,辽宁大连116034

年份：2024

卷号：42

期号：3

起止页码：92

中文期刊名：食品科学技术学报

外文期刊名：Journal of Food Science and Technology

收录：北大核心2023、CSTPCD、、EI(收录号：20242516287327)、Scopus、CSCD2023_2024、北大核心、CSCD

基金：国家重点研发计划课题(2018YFD0901105)。

语种：中文

中文关键词：龙趸石斑鱼;维生素A;形态分布;干眼症;细胞模型

外文关键词：Epinephelus drummondhayi;vitamin A;speciation distribution;xerophthalmia;cell model

中文摘要：为探究龙趸石斑鱼肝脏中天然维生素A的形态分布特征及其与合成维生素A对干眼症改善效果的差异,采用水浴皂化法结合反相色谱法测定龙趸石斑鱼肝脏各脂质组分中维生素A含量,并从中性脂中分离得到天然维生素A棕榈酸酯含量较高的F3组分,分别研究了F3组分与合成维生素A棕榈酸酯对苯扎氯铵诱导的干眼症细胞模型的影响及二者的差异。实验设置角膜上皮细胞正常组(NC)、干眼症细胞模型组(MC)、F3实验组[低剂量组(LF3)、中剂量组(MF3)、高剂量组(HF3)]及合成维生素A棕榈酸酯标准品对照组[低剂量组(LVAP)、中剂量组(MVAP)、高剂量组(HVAP)]。采用CC K-8法检测细胞活性及毒性,采用实时荧光定量法检测细胞内炎症因子(I L-6、I L-1β及TNF-α)的基因表达。结果表明:龙趸石斑鱼肝脏中99.57%的维生素A以结合态形式存在,主要是维生素A棕榈酸酯,且在肝脏中性脂中含量最多。从中性脂中分离纯化得到了含天然维生素A棕榈酸酯的F3组分,当F3组分质量浓度高于286.00 pg/mL时,HC E-T存活率显著下降。F3组分及合成维生素A棕榈酸酯均能显著提高苯扎氯铵诱导的干眼症细胞存活率,降低其凋亡率,且低、中剂量的F3组细胞活力高于高剂量F3组及低、中、高剂量的合成维生素A棕榈酸酯组。在白介素(I L-6)相对表达量中,低、中剂量的F3组及低剂量的合成维生素A棕榈酸酯组显著高于中、高剂量的合成维生素A棕榈酸酯组(P<0.05);在肿瘤坏死因子-α(TNF-α)相对表达量中,低、中、高剂量的F3组显著低于低剂量合成维生素A棕榈酸酯组(P<0.05)。F3组分及合成维生素A棕榈酸酯均能下调苯扎氯铵诱导的干眼症细胞模型中I L-6及TNF-α的基因表达(P<0.05),缓解干眼病导致的炎症。

外文摘要：To investigate the speciation distribution characteristics of natural vitamin A in the liver of Epinephelus drummondhayi and its difference in improve effects on xerophthalmia compared with synthetic vitamin A,the saponification method combined with reverse-phase chromatography was used to determine the vitamin A components in the liver lipids of Epinephelus drummondhayi.F3,a fraction rich in natural vitamin A palmitate,was isolated from the neutral lipids.Using synthetic vitamin A palmitate as a control,the effects of natural vitamin A palmitate from Epinephelus drummondhayi liver on a benzalkonium chloride-induced xerophthalmia cell model and their differences were explored.The experiment included normal corneal epithelial cell group(NC),xerophthalmia cell model group(MC),F3 experimental group[low dose(LF3),medium dose(MF3),and high dose(HF3)],and vitamin A palmitate standard control group[low dose(LVAP),medium dose(MVAP),and high dose(HVAP)].CC K-8 method was used to assess cell viability and toxicity,and real-time quantitative PCR was used to measure the gene expression of inflammatory factors(I L-6,I L-1β,and TNF-α)in cells.The results showed that 99.57%of vitamin A in Epinephelus drummondhayi liver existed in a bound form,mainly as vitamin A palmitate,with the highest content in the liver neutral lipids.The F3 fraction,isolated and purified from the neutral lipids,contained natural vitamin A palmitate,and when the mass concentration of F3 exceeded 286.00 pg/mL,the cell viability of HC E-T cells significantly decreased.Both F3 and synthetic vitamin A palmitate significantly improved the cell survival rate induced by benzalkonium chloride in the xerophthalmia cell model,reduced cell apoptosis,and the cell viability of the low and medium dose F3 group was higher than that of the high dose F3 group and all doses of synthetic vitamin A palmitate group.In terms of the relative expression level of interleukin(I L-6),the low and medium dose F3 group and the low dose synthetic vitamin A palmitate group were significantly higher than the medium and high dose synthetic vitamin A palmitate group(P<0.05).In the relative expression level of tumor necrosis factor-alpha(TNF-α),the low,medium,and high dose F3 groups were significantly lower than the low dose synthetic vitamin A palmitate group(P<0.05).Both F3 and synthetic vitamin A palmitate could downregulate the gene expression of I L-6 and TNF-αin HC E-T cells of the benzalkonium chloride-induced xerophthalmia cell model(P<0.05),alleviating the inflammation caused by xerophthalmia.