文献类型：期刊文献

英文题名：Protective effects of taurocholic acid on excessive hepatic lipid accumulation via regulation of bile acid metabolism in grouper

作者：Xu, Jia[1];Xie, Shiwei[1,2,3];Chi, Shuyan[1,2,3];Zhang, Shuang[1,2,3];Cao, Junming[1,2,3];Tan, Beiping[1,2,3]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Lab Aquat Anim Nutr & Feed, Zhanjiang, Peoples R China;[2]Aquat Anim Precis Nutr & High Efficiency Feed Eng, Zhanjiang, Peoples R China;[3]Minist Agr, Key Lab Aquat Livestock & Poultry Feed Sci & Tech, Zhanjiang, Peoples R China

年份：2022

卷号：13

期号：5

起止页码：3050

外文期刊名：FOOD & FUNCTION

收录：SCI-EXPANDED(收录号：WOS:000760408500001)、、WOS

基金：We sincerely thank Xiaohui Dong, Qihui Yang and Hongyu Liu (Laboratory of Aquatic Animal Nutrition and Feed of Fisheries College of Guangdong Ocean University of Zhanjiang in China) for providing methodological help during the experiments and proofreading the article. We would like to acknowledge Suzhou Bionovogene for providing technical help. This work was supported by the National Key R&D Program of China [2019YFD0900200], the National Natural Science Foundation of China [no. 31772864], the China Agriculture Research System [CARS-47], the Science and Technology Project of Zhanjiang [200914094542882] and the Natural Science Foundation of Guangdong Province [2018A030313154 and 2020A1515011129].

语种：英文

外文摘要：Dietary bile acid (BA) supplementation can notably ameliorate fatty liver disease caused by high dietary lipids, but the mechanism behind this is poorly understood. The present study was aimed at gaining insight into how TCA (taurocholic acid sodium) reduced hepatic lipid accumulation via the regulation of bile acid metabolism. We explored BA metabolism in juvenile hybrid grouper (Epinephelus fuscoguttatus female x E. lanceolatus male). Three trials were: (1) fed the control, high lipid (HD) or gradient TCA diet; (2) fed a BA diet with or without antibiotics; and (3) injected with an agonist or antagonist of TGR5 (G protein-coupled bile acid receptor 1) and FXR (farnesoid X receptor). The results showed that the TCA diet (about 900 mg kg(-1)) significantly reduced lipid accumulation in the liver, thus improving liver health. The HD suppressed the abundance of bile-salt hydrolase (BSH) microbes, thus decreasing the concentration of unconjugated primary BAs. TCA administration altered the gut microbial composition and weakened the effects of the HD, thus increasing the level of unconjugated BAs. TCA treatment increased the transport and reabsorption of BAs by activating the TGR5 and FXR signaling pathways, and increased the BA pool size. Furthermore, the presence of microbiota in the intestine increased BA reabsorption and the BA pool size. Our study revealed that exogenous TCA alters the structure of intestinal microbiota and BA composition, then activated the FXR expression, thus regulating the BA metabolism via enhanced BA reabsorption. This, in turn, reduced lipid accumulation and improved the health of the liver in grouper.