文献类型：期刊文献

中文题名：不同海洋生物源肝素的理化性质及抗凝血活性

英文题名：Physicochemical Properties and Anticoagulant Activity of Heparin from Different Marine Organisms

作者：杜振兴[1,2,3];周斯仪[1,2,3];钟赛意[1,2,3,4];陈观兰[1,2,3];陈建平[2,3];谌素华[2,3];洪鹏志[1,2,3,4]

机构：[1]广东海洋大学深圳研究院,广东深圳518108;[2]广东海洋大学食品科技学院,广东湛江524088;[3]广东省水产品加工与安全重点实验室,广东湛江524088;[4]广东省海洋生物制品工程实验室,广东湛江524088

年份：2019

卷号：40

期号：17

起止页码：134

中文期刊名：食品科学

外文期刊名：Food Science

收录：CSTPCD、、EI(收录号：20194207561125)、北大核心2017、Scopus、CSCD_E2019_2020、北大核心、CSCD

基金：深圳市科技计划项目（JCYJ20170818111335796）;广东省渔港建设和渔业产业发展专项（A201708C13）;湛江市海洋经济创新发展示范市建设项目（2017C8B1）;广东普通高等学校水产品高值化加工与利用创新团队项目（GDOU2016030503）

语种：中文

中文关键词：肝素;海洋生物;理化性质;结构鉴定;抗凝血活性

外文关键词：heparin;marine organisms;physicochemical properties;structural identification;anticoagulant activity

中文摘要：为研究和开发海洋生物源肝素,本实验采用酶解醇沉法从9种海洋生物中提取出粗肝素,采用紫外光谱法、傅里叶变换红外光谱法和高效液相色谱法对其理化性质和结构特性进行分析,并通过体外凝血实验分析其抗凝血活性。结果表明:9种肝素粗品的肝素含量从高到低依次为凡纳滨对虾(Penaeus vannaei)虾头339.92 g/mg、海蚌(Mactra antiquata)239.15 g/mg、泥蚶(Tegillarca granosa)234.85 g/mg、蛏子(Sinonovacula constricta)218.29 g/mg、文蛤(Meretrix meretrix L.)214.78 g/mg、钝缀锦蛤(Tapes dorsatus)212.194 g/mg、海湾扇贝(Argopecten irradias)68.52 g/mg、象拔蚌(Panopea abrupta)61.48 g/mg、菲律宾蛤蜊(Ruditapes philippinarum)24.29 g/mg,其均为吡喃糖,且都含有肝素的主要单糖:氨基葡萄糖、艾杜糖醛酸和葡萄糖醛酸。体外凝血实验结果表明,9种粗肝素效价从高到低依次为海蚌67.1 U/mg、凡纳滨对虾虾头59.3 U/mg、文蛤44.0 U/mg、钝缀锦蛤39.2 U/mg、蛏子35.0 U/mg、泥蚶29.6 U/mg、象拔蚌27.2 U/mg、海湾扇贝27.1 U/mg、菲律宾蛤蜊24.1 U/mg,它们具有不同强度的抗凝血活性,且发挥抗凝血作用的途径不同。实验结果可为海洋源肝素的开发提供参考。

外文摘要：In this study,crude heparin was extracted from 9 marine organisms by enzymatic hydrolysis and then alcohol precipitation.The physicochemical properties and monosaccharide composition of the heparins were analyzed by ultraviolet spectroscopy,Fourier transform infrared spectroscopy and high performance liquid chromatography,and their anticoagulant activities were analyzed by in vitro experiments.The results showed that the heparin contents of the 9 crude heparin samples were 339.92 g/mg for Penaeus vannaei heads,239.15 g/mg for Mactra antiquata,234.85 g/mg for Tegillarca granosa,218.29 g/mg for Sinonovacula constricta,214.78 g/mg for Meretrix meretrix L.,212.194 g/mg for Tapes dorsatus,68.52 g/mg for Argopecten irradias,61.48 g/mg for Panopea abrupta,and 24.29 g/mg for Ruditapes philippinarum.All of them,having pyranose rings,contained glucosamine,iduronic acid,and galacturonic acid.The in vitro experiments showed that the titers of the crude heparin samples were 67.1 U/mg for Mactra antiquata,59.3 U/mg for Penaeus vannaei heads,44.0 U/mg for Meretrix meretrix L.,39.2 U/mg for Tapes dorsatus,35.0 U/mg for Sinonovacula constricta,29.6 U/mg for Tegillarca granosa,27.2 U/mg for Panopea abrupta,27.1 U/mg for Argopecten irradias,and 24.1 U/mg for Ruditapes philippinarum.They exerted different anticoagulant activities through different pathways.This study provides a basis for the development of heparin from marine resources.