文献类型：期刊文献

中文题名：蚯蚓粪有机肥对农田土壤磺胺甲噁唑去除性能及微生物群落结构的影响

英文题名：Effects of vermicompost organic fertilizer on sulfamethoxazole removal performance and microbial community structure in farmland soil

作者：尹俊泳[1];杨贵琼[1];蔺中[2];李进[2];李哲[3];白婧[4];李金成[5];甄珍[1]

机构：[1]广东海洋大学滨海农业学院,广东湛江524088;[2]广东海洋大学化学与环境学院,广东湛江524088;[3]齐鲁工业大学(山东省科学院)生物研究所,山东济南250014;[4]湖南人文科技学院农业与生物技术学院,湖南娄底417000;[5]湛江市慧鹏农业生态科技有限公司,广东湛江524000

年份：2026

卷号：57

期号：4

起止页码：1062

中文期刊名：南方农业学报

外文期刊名：Journal of Southern Agriculture

收录：北大核心2023、、北大核心

基金：国家自然科学基金项目(41977125);广东省自然科学基金项目(2023A1515012225,2022A1515010867,2024A151501010766)。

语种：中文

中文关键词：农田土壤;蚯蚓粪有机肥;磺胺甲噁唑;酶活性;微生物群落结构

外文关键词：farmland soil;vermicompost organic fertilizer;sulfamethoxazole;enzyme activity;microbial community structure

中文摘要：【目的】研究不同剂量蚯蚓粪有机肥对农田土壤中磺胺甲噁唑的降解效果及微生物群落结构变化的影响,获得最佳降解效果的蚯蚓粪有机肥剂量和降解微生物种类,为优化磺胺甲噁唑污染农田土壤的修复效果提供理论依据。【方法】通过添加不同剂量(1%、2%、3%、4%)的蚯蚓粪有机肥,明确农田土壤修复过程中磺胺甲噁唑的去除效率、土壤理化性质、酶活性和土壤微生物的变化情况,并分析磺胺甲噁唑残留浓度与环境因子、土壤微生物的相关性。【结果】蚯蚓粪有机肥主要通过提高pH、有机质含量和腐殖质含量进而加速农田土壤中磺胺甲噁唑的降解,其中添加2%蚯蚓粪有机肥的效果最优,磺胺甲噁唑降解率达82.8%。培养周期结束后,添加2%蚯蚓粪有机肥处理的土壤pH为6.51,有机质含量为21.08 g/kg,腐殖质含量为14.52 g/kg,分别较未添加蚯蚓粪有机肥处理(CK)提高7.43%、3.84%和11.35%。同时,蚯蚓粪有机肥能提升土壤过氧化氢酶、脲酶和脱氢酶活性,并促进磺胺甲噁唑降解微生物类诺卡氏菌科(Nocardioidaceae)、甲基杆菌科(Methylobacteriaceae)、间孢囊菌科(Intrasporangiaceae)、黄色杆菌科(Xanthobacteraceae)、地杆菌科(Geobacteraceae)、小单胞菌科(Micromonosporaceae)、丛毛单胞菌科(Comamonadaceae)的相对丰度提升。相关性热图显示,甲基杆菌科、类诺卡氏菌科、间孢囊菌科、地杆菌科、硝化杆菌科(Nitrobacteraceae)、芽单胞菌科(Gemmatimonadaceae)、pH、有机质、腐殖质、脲酶、过氧化氢酶、脱氢酶与磺胺甲噁唑残留浓度均呈负相关。【结论】在磺胺甲噁唑污染农田土壤中施入蚯蚓粪有机肥,可通过改善土壤理化性质(p H、有机质、腐殖质)和土壤酶活性,提高磺胺甲噁唑降解微生物的相对丰度,进而促进农田土壤中磺胺甲噁唑的去除,其中添加2%蚯蚓粪有机肥的作用效果最佳。

外文摘要：【Objective】This study aimed to investigate the effects of different vermicompost organic fertilizer doses on sulfamethoxazole degradation and the changes in microbial community structure in farmland soil,to screen the optimal dose of vermicompost organic fertilizer for the best degradation effect and types of degrading microorganisms,providing theoretical basis for optimizing the remediation effect of sulfamethoxazole-contaminated farmland soil.【Method】Different doses(1%,2%,3%,4%)of vermicompost organic fertilizer were added to investigate the sulfamethoxazole removal efficiency,soil physicochemical properties,enzyme activities,and the changes in soil microorganisms during the remediation of farmland soil,and analyze the correlations between sulfamethoxazole residual concentration,environmental factors,and soil microorganisms.【Result】By increasing pH,organic matter content,and humus content,vermicompost organic fertilizer accelerated sulfamethoxazole degradation in farmland soil.Adding 2%vermicompost organic fertilizer yielded the best effect,as the sulfamethoxazole degradation rate reaching 82.8%.After the end of the culture period,the pH was 6.51,the organic matter content was 21.08 g/kg,and the humus content was 14.52 g/kg when adding 2%vermicompost organic fertilizer,showing increases of 7.43%,3.84%,and 11.35%compared with the vermicompostfree treatment(CK)respectively.Meanwhile,vermicompost organic fertilizer enhanced the activities of peroxidase,urease,and dehydrogenase in soil,and promoted the increase in abundance of the potential sulfamethoxazole-degrading microorganisms,such as Nocardioidaceae,Methylobacteriaceae,Intrasporangiaceae,Xanthobacteraceae,Geobacteraceae,Micromonosporaceae,and Comamonadaceae.Correlation heatmap analysis indicated that Methylobacteriaceae,Nocardioidaceae,Intrasporangiaceae,Geobacteraceae,Nitrobacteraceae,Gemmatimonadaceae,pH,organic matter,humus,urease,catalase,and dehydrogenase were negatively correlated with sulfamethoxazole residual concentrations.【Conclusion】Applying vermicompost organic fertilizer to sulfamethoxazole-contaminated farmland soil can improve soil physicochemical properties(pH,organic matter,and humus)and soil enzyme activity to increase the relative abundance of sulfamethoxazole-degrading microorganisms,promoting the sulfamethoxazole removal in farmland soil,with adding 2%vermicompost organic fertilizer yielding the best effect.