文献类型：期刊文献

中文题名：2株大白鲨鳃部来源青霉生物碱及其生物活性研究

英文题名：Study on the alkaloids from two great white shark's gill-derived Penicillium strains and their bioactivity

作者：冯妍[1];韩金媛[2];张翼[1,3];苏煦[2];ESSMANN Frank[3];GROND Stephanie[3]

机构：[1]广东海洋大学分析测试中心,食品科技学院,广东省水产品加工与安全重点实验室,广东省高校水产品深加工重点实验室,广东湛江524088;[2]大连交通大学环境与化学工程学院,辽宁大连116028;[3]Tübingen大学有机化学研究所、生物化学多学科研究所,德国Tübingen72076

年份：2016

卷号：35

期号：4

起止页码：16

中文期刊名：中国海洋药物

外文期刊名：Chinese Journal of Marine Drugs

收录：CSTPCD、、CSCD2015_2016、CSCD

基金：国家自然科学基金项目(20902009);广东省扬帆计划引进紧缺拔尖人才项目(201433009);广东海洋大学科研启动项目(E15155);广东海洋大学创新强校项目(GDOU2014030502,GDOU2014050203)资助

语种：中文

中文关键词：鲨鱼;真菌;青霉;生物碱;细胞毒

外文关键词：sharks; fungi; Penicillium; alkaloids; cytotoxicity

中文摘要：目的探索大白鲨鳃部共附生真菌菌株Penicilliumsp.BP2T2和Penicilliumsp.TBG2-2的次生代谢产物。方法利用柱色谱和高效液相色谱对发酵产物进行分离纯化,综合运用质谱、一维与二维核磁共振谱、比旋光测定和圆二色谱来鉴定化合物结构,采用结晶紫法评价化合物细胞毒活性,采用改良的微孔板法评价卤虫Artemia salina致死活性,采用纸片法评价其抗菌活性。结果从菌株Penicilliumsp.BP2T2发酵产物中分离鉴定到1个生物碱Meleagrin（1）,从菌株Penicilliumsp.TBG2-2发酵产物中分离鉴定到3个生物碱,分别为fructigenine A（2）、（-）-cyclopenol（3）和viridicatol（4）。化合物1、2和4显示了对人结肠癌HCT116细胞株的细胞毒性,其半抑制浓度（IC50）分别为5.1、40.5和59.0μg/mL,并显示了对金黄色葡萄球菌或大肠埃希氏菌弱的抑菌活性,25μg/片剂量下抑菌圈直径为7.5~8.0mm。化合物2对卤虫的24h半致死浓度为125μg/mL。结论首次报道从鲨鱼鳃真菌中分离得到化合物1~3以及化合物1、2和4对HCT116细胞株的细胞毒性,鲨鱼鳃真菌作为研究较少的真菌资源在产生生物碱等次生代谢产物方面具有一定潜力。

外文摘要：Objective To investigate the secondary metabolites from two symbiotic fungi Penicilliumsp.BP2T2 and Penicillium sp.TBG2-2isolated from the gill of the Carcharodon carcharias（the great white shark）.Methods The isolation and purification of compounds were performed by combined use of column chromatographies and HPLC.Their structures were elucidated by comprehensive analyses of MS,1D-and 2D-NMR,specific optical rotation,and ECD spectra.Their cytotoxicity was evaluated by crystal violet method,their brine shrimp lethality towards Artemia salina was tested by improved microwell test method,and their antimicrobial activity was assayed by paper disk diffusion method and micro-dilution method.Results One alkaloid meleagrin（1）was characterized fromPenicilliumsp.BP2T2,and 3alkaloids fructigenine A（2）,（-）-cyclopenol（3）and viridicatol（4）were characterized fromPenicilliumsp.TBG2-2.Preliminary studies showed that 1,2 and 4 had moderate to weak cytotoxicity against HCT116 human colon cancer cell line with IC50 values of 5.1μg/mL,40.5μg/mL and 59.0μg/ml,respectively.Besides,compounds 1,2 and 4 also displayed weak inhibition against Staphylococcus aureus or Escherichia coli with 7.5～8.0mm inhibition zone at the doze of 25μg/disk.Compound 2 was able to kill the brine shrimp larvae with 24h-LC50 of 125μg/mL.Conclusion It was the first time to report the isolation of compounds 1–3from shark-derived fungi and the cytotoxictiy of compounds 1,2 and 4towards HCT116 cell line.As a less explored resource,shark-derived fungi showed potential in producing secondary metabolites like alkaloids.