文献类型：期刊文献

英文题名：PmRunt regulated by Pm-miR-183 participates in nacre formation possibly through promoting the expression of collagen VI-like and Nacrein in pearl oyster Pinctada martensii

作者：Zheng, Zhe[1];Du, Xiaodong[1,2];Xiong, Xinwei[1];Jiao, Yu[1];Deng, Yuewen[1];Wang, Qingheng[1];Huang, Ronglian[1]

机构：[1]Guangdong Ocean Univ, Fishery Coll, Zhanjiang, Peoples R China;[2]Guangdong Ocean Univ, Ctr Pearl Aquaculture & Proc, Guangdong Technol Res, Zhanjiang, Peoples R China

年份：2017

卷号：12

期号：6

外文期刊名：PLOS ONE

收录：SCI-EXPANDED(收录号：WOS:000402611800077)、、Scopus(收录号：2-s2.0-85020025424)、WOS

基金：The studies were financially supported by grants of the National Natural Science Foundation of China (31272635, 31372526), Natural Foundation of Guangdong Ocean University (1212318), Excellent Young Scientist Foundation of Guangdong Ocean University (201402), Excellent Doctoral Dissertation Breeding Project of Guangdong Ocean University (201601).; The studies were financially supported by grants of the National Natural Science Foundation of China (31272635, 31372526), Natural Foundation of Guangdong Ocean University (1212318), Excellent Young Scientist Foundation of Guangdong Ocean University (201402), Excellent Doctoral Dissertation Breeding Project of Guangdong Ocean University (201601).

语种：英文

外文摘要：Heterodimeric PEBP2/CBFs are key regulators in diverse biological processes, such as haematopoietic stem-cell generation, bone formation and cancers. In this work, we cloned runt-like transcriptional factor (designated as PmRunt) and CBF beta (designated as PmCBF) gene, which comprise the heterodimeric transcriptional factor in Pinctada martensii. PmRunt was identified with an open reading frame that encodes 545 amino acids and has typical Runt domain. Phylogenetic analysis results speculated that runt-like transcriptional factors (RDs) in vertebrates and invertebrates are separated into two branches. In molluscs, PmRunt and other RDs are clustered in one of these branches. Direct interaction between PmRunt and PmCBF was evidenced by yeast two-hybrid assay results. Gene repression by RNA interference decreased the expression level of PmRunt, and subsequent observation of the inner surface of the nacre by scanning electron microscopy demonstrated disordered growth. The luciferase activities of reporters that contain promoter regions of Collagen VI-like (PmColVI) and PmNacrein were enhanced by PmRunt. Meanwhile, Pm-miR-183 apparently inhibited the relative luciferase activity of reporters containing the 3'-UTR of PmRunt. The expression level of PmRunt was repressed after Pm-miR-183 was overexpressed in the mantle tissue. Therefore, we proposed that PmRunt could be targeted by Pm-miR-183 and regulate the transcription of PmColVI and PmNacrein by increasing their transcriptional activity, thereby governing nacre formation.