文献类型：期刊文献

英文题名：Effects of dietary methionine on growth performance and metabolism through modulating nutrient-related pathways in largemouth bass (Micropterus salmoides)

作者：Wang, Wenqiang[1];Yang, Peng[1];He, Chaoqun[1];Chi, Shuyan[3];Li, Songlin[4];Mai, Kangsen[1];Song, Fei[1,2]

机构：[1]South China Normal Univ, Inst Modern Aquaculture Sci & Engn IMASE, Guangzhou Key Lab Subtrop Biodivers & Biomonitori, Coll Life Sci,Guangdong Prov Key Lab Hlth & Safe, Guangzhou 510631, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China;[3]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Anim Nutr & Feed, Zhanjiang 524088, Guangdong, Peoples R China;[4]Shanghai Ocean Univ, Minist Agr & Rural Affairs Environm Ecol & Fish N, Res Ctr, Shanghai 20136, Peoples R China

年份：2021

卷号：20

外文期刊名：AQUACULTURE REPORTS

收录：SCI-EXPANDED(收录号：WOS:000672568200006)、、Scopus(收录号：2-s2.0-85101377310)、WOS

基金：This work was supported by the Foundation and Applied Basic Research Fund project of Guangdong Province (2019A1515110606), Research and Development Fund for Young Teachers of South China Normal University (19KJ03) and the Research Start-Up Fund of South China Normal University.

语种：英文

外文关键词：Methionine; TOR; AAR; GH-IGF; Largemouth bass (Micropterus salmoides)

外文摘要：Methionine (Met) as molecular signaling plays a vital regulatory role in growth-related cellular functions. However, more research in the regulatory mechanism of Met in fish species is needed. Therefore, this study was designed to elucidate the regulatory role of dietary Met on body growth performance and metabolism. Largemouth bass (initial body weight: 4.10 +/- 0.01 g) fed with control (CON, Met: 1.21 % of diet) diet and Met deficiency (MD, Met: 0.93 % of diet) diet, respectively for 8 weeks. Results of present study showed that compared with the MD diet (specific growth performance: 3.01 +/- 0.03 %/d; weight gain ratio: 438 +/- 9.16 %), control diet significantly increased the specific growth performance (3.36 +/- 0.02 %/d) and weight gain ratio (557 +/- 7.41 %), respectively (P < 0.05). Moreover, the fish fed with control diet also had higher feed efficient ratio and protein efficiency ratio (1.12 +/- 0.01; 2.28 +/- 0.01) than the fish fed with MD diet (0.88 +/- 0.03; 1.83 +/- 0.05). Mechanically, present study demonstrated that Met supplementation increased the amino acid (AA) concentrations through improving transport efficiency of AA transporters. Elevated AA concentrations promoted systemic nutrient sensing by activating the growth hormone-insulin-like growth factor (GH-IGF) signaling pathway. Activated GH-IGF signaling then up-regulated the target of rapamycin (TOR) signaling and suppressed the amino acids response (AAR) signaling. Up-regulated TOR and down-regulated AAR caused by Met supplementation acted together to increase the nutrient metabolic level, which further accelerate the growth performance. Our study better explained how fish through nutrient sensing signaling to coordinating growth and metabolism in response to dietary Met availability.