文献类型：期刊文献

英文题名：Structural characterization and ferroptosis-related immunomodulatory of a novel exopolysaccharide isolated from marine fungus Aspergillus medius

作者：Yang, Jiajia[1];Liu, Jun[2];Kuang, Weiyang[1];Lin, Yuqi[1];Zhong, Saiyi[3];Kraithong, Supaluck[1];Zhang, Xiaoyong[4];Wong, Io Nam[5];Huang, Riming[1]

机构：[1]South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Peoples R China;[2]Guangdong Med Univ, Lab Pathogen Biol, Zhanjiang 524023, Peoples R China;[3]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Peoples R China;[4]South China Agr Univ, Coll Marine Sci, Joint Lab Guangdong Prov & Hong Kong Reg Marine Bi, Guangzhou 510642, Peoples R China;[5]Macau Univ Sci & Technol, Fac Med, Cotai 999078, Macao, Peoples R China

年份：2024

卷号：265

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：SCI-EXPANDED(收录号：WOS:001221105900001)、、EI(收录号：20241315812111)、Scopus(收录号：2-s2.0-85188535486)、WOS

基金：This work was supported by the National Natural Science Foundation of China (No. 32161160304 & No. 32272241), the Characteristic Innovation Project of Colleges and Universities in Guangdong Province (No. 2020KTSCX041), the general project of Guangdong Medical Science and Technology Research Foundation (No. A2021207), the Finance Special Project of Zhanjiang City (No. 2021A05095), the Macao Science and Technology Development Fund (No. 0069/2021/AFJ), the MUST Faculty Research Grants (No. FRG-22-022-FMD), the Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety (No. GDPKLAPPS2304).

语种：英文

外文关键词：Aspergillus medius; Polysaccharide; Structural characterization; Immunostimulatory effect; Ferroptosis

外文摘要：Marine fungal exopolysaccharides play a crucial role in immunoregulation. In this investigation, a novel polysaccharide was extracted from the culture medium of the marine fungus Aspergillus medius SCAU-236. Compositional analysis revealed a structure composed of glucose units with (1,4)- alpha-D-Glc p , (1,3,4)- beta-D-Glc p , and (1,4,6)- alpha-D-Glc p , along with side chains of 1- alpha-D-Glc p linked to carbon 6 of (1,4,6)- alpha-D-Glc p and carbon 3 of (1,3,4)- beta-D-Glc p . Functional evaluations on RAW264.7 macrophage cells demonstrated Aspergillus medius polysaccharide (ASMP)'s effects on cell proliferation, nitric oxide levels, and the secretion of TNF- alpha, IL -6, and IL-1 beta cytokines. Additionally, metabolomics indicated ASMP's potential to modulate macrophage immune function by impacting key regulatory molecules, including COX -2, iNOS, Nrf2, SLC7A11, GPX4, and ACSL4. The Nrf2/ SLC7A11/GPX4 axis and ACSL4 were suggested to be involved in ASMP-induced ferroptosis, leading to increased reactive oxygen species (ROS) levels and lipid peroxidation. These findings propose a unique mechanism by which ASMP exerts immunomodulatory effects through ferroptosis induction, contributing to the understanding of marine -derived compounds in immunomodulation research.