文献类型：期刊文献

中文题名：产细菌素贝莱斯芽孢杆菌CM7-4全基因组测序及抑菌活性研究

英文题名：Whole Genome Sequencing of Bacteriocin-producing Bacillus velezensis CM7-4 and Its Bacteriostatic Activity

作者：马浩天[1];李杨[1];余梦博[1];潘瑞雪[1];龙赟而[1];赵一宁[1];彭金菊[1];马驿[1]

机构：[1]广东海洋大学滨海农业学院,湛江524000

年份：2024

卷号：51

期号：12

起止页码：5117

中文期刊名：中国畜牧兽医

外文期刊名：China Animal Husbandry & Veterinary Medicine

收录：北大核心2023、CSTPCD、、CSCD_E2023_2024、北大核心、CSCD

基金：广东省自然科学基金项目(2023A1515012181)。

语种：中文

中文关键词：芽孢杆菌;全基因组测序;细菌素;基因功能注释

外文关键词：Bacillus;whole genome sequencing;bacteriocin;gene function annotation

中文摘要：【目的】贝莱斯芽孢杆菌(Bacillus velezensis)CM7-4是从海水中分离的具有良好抗菌活性的芽孢杆菌。本试验旨在探究贝莱斯芽孢杆菌CM7-4的抗菌机理,并对其全基因组序列进行分析,以期挖掘其抗菌等功能特性基因。【方法】通过酸沉淀法得到贝莱斯芽孢杆菌CM7-4的细菌素粗提物,通过固体琼脂打孔法测定蛋白酶K对其抑菌效果的影响;基于MGI DNBSEQ-T7平台对贝莱斯芽孢杆菌CM7-4进行全基因组测序分析,通过序列组装、基因预测和功能注释分析其产细菌素的潜质。【结果】贝莱斯芽孢杆菌CM7-4的细菌素粗提物对大肠杆菌、沙门菌、粪肠球菌、单增李斯特菌等8种不同的革兰阴性和阳性菌具有良好的抑菌效果;与对照组相比,经蛋白酶K处理后细菌素粗提物对8种指示菌的抑菌效果均极显著下降(P<0.01);全基因组测序显示,贝莱斯芽孢杆菌CM7-4的基因组大小为3953124 bp,GC含量为47.04%;共预测到3870个蛋白编码基因、9个rRNA、81个tRNA、1个ncRNA;基因组中含有11个与细菌素合成相关的基因、10个与抗氧化活性相关基因,以及4个与调控免疫和炎症信号通路相关的基因。【结论】贝莱斯芽孢杆菌CM7-4是一株潜在的产细菌素菌株,为进一步研究其抗菌特性提供了理论基础。

外文摘要：【Objective】Bacillus velezensis CM7-4 was isolated from seawater and had been demonstrated to possess good antibacterial activity.This study was to investigate the antimicrobial mechanism of Bacillus velezensis CM7-4 and analyze the whole genome sequence in order to explore its antibacterial and other functional genes.【Method】The bacteriocin crude extract of Bacillus velezensis CM7-4 was obtained by acid precipitation,and the effect of proteinase K on its antibacterial effect was determined by the solid agar perforation method.Subsequently,the complete genome of Bacillus velezensis CM7-4 was sequenced on the MGI DNBSEQ-T7 platform.Their bacteriocin production potential was analyzed by sequence assembly,gene prediction and functional annotation.【Result】The bacteriocin crude extract of Bacillus velezensis CM7-4 demonstrated a notable bacteriostatic effect on a diverse range of 8 kinds of Gram-negative and positive bacteria,including Escherichia coli,Salmonella,Enterococcus faecalis,Listeria monocytogenes and others.Compared with control group,the bacteriocin crude extract demonstrated a extremely significantly diminished inhibitory effect on the eight indicator bacteria following treatment with proteinase K(P<0.01).Whole genome sequencing revealed that the genome size of Bacillus velezensis CM7-4 was 3953124 bp with a 47.04%GC content.A total of 3870 protein-coding genes,9 rRNA,81 tRNA and 1 ncRNA were identified.And 11 bacteriocin synthesis genes,10 antioxidant activity genes,and 4 immune and inflammatory signalling pathway regulation genes were predicted.【Conclusion】The results of this experiment indicated that Bacillus velezensis CM7-4 was a potential bacteriocin-producing strain.This study provided a theoretical basis for further study of its antibacterial properties.