文献类型：期刊文献

英文题名：Combined effects of water temperature and copper ion concentration on catalase activity in Crassostrea ariakensis

作者：Wang Hui[1];Yang Hongshuai[2];Liu Jiahui[2];Li Yanhong[2];Liu Zhigang[2]

机构：[1]Huaiyin Normal Univ, Sch Life Sci, Huaian 223300, Peoples R China;[2]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China

年份：2015

卷号：33

期号：4

起止页码：905

外文期刊名：CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000357037100011)、、WOS

基金：Supported by the Guangdong Province Science & Technology Project (No. 2010B020201014), the Guangdong Province Education Department (No. GCZX-A0909), the Guangdong Province Ocean and Fisheries Science & Technology Extension Project (No. 20120980), the Guangdong Province Industry-University-Science Partnership Project (No. 20110908), and the Sci & Tech Plan of Huaiyin Normal University (No. 00wh0031)

语种：英文

外文关键词：Crassostrea ariakensis; catalase; temperature; copper ion concentration; combined effects

外文摘要：A central composite experimental design and response surface method were used to investigate the combined effects of water temperature (18-34A degrees C) and copper ion concentration (0.1-1.5 mg/L) on the catalase (CAT) activity in the digestive gland of Crassostrea ariakensis. The results showed that the linear effects of temperature were significant (P < 0.01), the quadratic effects of temperature were significant (P < 0.05), the linear effects of copper ion concentration were not significant (P > 0.05), and the quadratic effects of copper ion concentration were significant (P < 0.05). Additionally, the synergistic effects of temperature and copper ion concentration were not significant (P > 0.05), and the effect of temperature was greater than that of copper ion concentration. A model equation of CAT enzyme activity in the digestive gland of C. ariakensis toward the two factors of interest was established, with R (2), Adj. R (2) and Pred. R (2) values as high as 0.943 7, 0.887 3 and 0.838 5, respectively. These findings suggested that the goodness of fit to experimental data and predictive capability of the model were satisfactory, and could be practically applied for prediction under the conditions of the study. Overall, the results suggest that the simultaneous variation of temperature and copper ion concentration alters the activity of the antioxidant enzyme CAT by modulating active oxygen species metabolism, which may be utilized as a biomarker to detect the effects of copper pollution.