文献类型：期刊文献

英文题名：Phoenixin: Expression at different ovarian development stages and effects on genes ralated to reproduction in spotted scat, Scatophagus argus

作者：Wang, Mei[1,2];Chen, Hua-Pu[1,2];Zhai, Yi[1,2];Jiang, Dong-neng[1,2];Liu, Jian-Ye[1,2];Tian, Chang-Xu[1,2];Wu, Tian-li[1,2];Zhu, Chun-Hua[1,2];Deng, Si-Ping[1,2,3];Li, Guang-Li[1,2]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Guangdong Res Ctr Reprod Control & Breeding Techn, Zhanjiang 524088, Peoples R China;[3]Marine Ecol & Aquaculture Environm Zhanjiang, Zhanjiang 524088, Peoples R China

年份：2019

卷号：228

起止页码：17

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000456639400003)、、WOS

基金：This study was supported by Natural Science Foundation of Guangdong Province (2016A030313743, 2017A030313101); Marine Fishery Science and Technology Extension Projects of Guangdong (A201608B01); The National Natural Science Foundation of China (Nos. 41706174, 31702326); Zhanjiang Science and Technology Bureau (2016A03017).

语种：英文

外文关键词：Scatophagus argus; Phoenixin-14; Phoenixin-20; Reproduction

外文摘要：Phoenixin (Pnx), a recently discovered neuropeptide, has been implicated in reproduction. Pnx mainly exists in two active isoforms, phoenixin-14 (Pnx-14) and phoenixin-20 (Pnx-20). However, little is known about the functions of Pnx in teleosts. To determine the roles of Pnx in the regulation of reproduction in Scatophagus argus, the physiological characterization of the Pnx was analyzed. During ovary development, the expression of pnx in phase IV was higher than in phase II and III in the hypothalamus. In the pituitary, pnx expression was highest in phase IV, moderate in phase III, and lowest in phase II. When hypothalamus and pituitary fragments were cultured in vitro with Pnx-14 and Pnx-20 (10 nM and 100 nM) for 6 h, the expression of GnRHR (gonadotropin releasing hormone receptor), lh (luteinizing hormone) and fsh (follicular stimulating hormone) in the pituitary increased significantly, except GnRH (gonadotropin releasing hormone) in the hypothalamus. Similarly, the expression of GnRHR, lh and fsh in the pituitary increased significantly after injecting S. argus with Pnx-14 and Pnx-20 (10 ng/g and 100 ng/g body weight (bw)), except GnRHR and fsh treated with 10 ng/gbw Pnx-20 in the pituitary and GnRHs in the hypothalamus. These results indicate that Pnx may not only stimulate the reproduction of the S. argus through the hypothalamic-pituitary-gonadal (HPG) axis, but also directly through the pituitary.