文献类型：期刊文献

英文题名：Short-term exposure to norethisterone affected swimming behavior and antioxidant enzyme activity of medaka larvae, and led to masculinization in the adult population

作者：Dong, Zhongdian[1,2];Li, Xueyou[1];Chen, Yuebi[1];Zhang, Ning[1];Wang, Zhongduo[1,4];Liang, Yan-Qiu[3];Guo, Yusong[1]

机构：[1]Guangdong Ocean Univ, Key Lab Aquaculture South China Sea Aquat Econ Ani, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Fisheries Coll, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China;[3]Guangdong Ocean Univ, Fac Chem & Environm Sci, Zhanjiang 524088, Peoples R China;[4]Hunan Normal Univ Sch, Coll Life Sci, State Lab Dev Biol Freshwater Fish, Changsha 410081, Peoples R China

年份：2023

卷号：310

外文期刊名：CHEMOSPHERE

收录：SCI-EXPANDED(收录号：WOS:000916172300004)、、EI(收录号：20224312994700)、Scopus(收录号：2-s2.0-85140051451)、WOS

基金：This work was supported by the National Natural Science Foundation of China (41806195, 31972794) ; Natural Science Foundation of Guangdong Province (2022A1515012107, 2022A1515011441) Guang- dong -Guangxi Joint Fund (2020A1515410009) ; Nanhai Scholar Project of Guangdong Ocean University (QNXZ201903, QNXZ201807)

语种：英文

外文关键词：Oryzias melastigma; Norethisterone; RNA-Seq; Synthetic progestins; Behavior; Masculinization

外文摘要：Norethisterone (NET), one of the synthetic progestins, is detected with increasing frequency in the water envi-ronment and distributed in the ocean, with a potential toxicity risk to marine organisms. However, current studies on the adverse effects of progestins (including NET) in aquatic environments have focused on freshwater organisms, mainly fish. In the present, marine medaka (Oryzias melastigma) larvae were exposed to 91.31 ng/L NET for 10 days, and then the swimming behavior, oxidation-antioxidant-related enzyme activities, sex and thyroid hormone levels, and the gene transcription patterns of the larvae were measured. After NET treatment, medaka larvae were raised in artificial seawater until 5 months of age, and the sex ratio was counted. Ten-day exposure to 91.31 ng/L NET inhibited swimming behavior, of marine medaka larvae, which showed that the time in the resting state was significantly prolonged, while the time in the large motor state was significantly reduced; disrupted oxidative-antioxidant system, significantly up-regulated the enzymatic activities of reactive oxygen species (ROS), malondialdehyde (MDA), and glutathione peroxidase (GSH-Px); affected the hormone levels of larvae, lowered 11-keto testosterone (11-KT) and triiodothyronine (T3) concentrations. RNA-seq results showed that 91.31 ng/L NET exposure for 10 days changed the transcript levels of 275 genes, of which 28 were up regulated and 247 were down-regulated. Differentially expressed genes (DEGs) were mainly significantly enriched in piwi interacting RNA (piRNA), gonadal development, gametogenesis, and steroidogenesis biological processes, etc. After removing NET exposure and returning to breeding for 140 days, a significant increase in male proportions (69.67%) was observed in sexually mature medaka populations in the NET-treated group. These results show that exposure to 91.31 ng/L NET for 10 days can lead to various adverse effects on marine medaka larvae. These findings shed light on the potential ecological risks of synthetic progestins to marine organisms.