文献类型：期刊文献

英文题名：Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Nocardia salmonicida, the Causative Agent of Nocardiosis in Fish

作者：Xia, Liqun[1,2,3];Zhang, Honglian[1];Lu, Yishan[1,2,3];Cai, Jia[1,2,3];Wang, Bei[1,2,3];Jian, Jichang[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524025, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China;[3]Key Lab Dis Controlling Aquat Econ Anim Guangdong, Zhanjiang 524088, Peoples R China

年份：2015

卷号：25

期号：3

起止页码：321

外文期刊名：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000351975800003)、、WOS

基金：This study was supported by the International S&T Cooperation Projects of Guangdong Province (2012B050600029), the Project of Internation as well as Hongkong, Macao & Taiwan Science and Technology Cooperation Innovation Platform in Universities in Guangdong Province (2013gjhz0008) and the Projects for the Innovation and Development in Higher Education of Guangdong Province (2014KTSCX075).

语种：英文

外文关键词：Loop-mediated isothermal amplification (LAMP); Nocardia salmonicida; rapid detection

外文摘要：Nocardia salmonicida is one of the main pathogens of fish nocardiosis. The purpose of this study was to build a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of N. salmonicida. A set of four primers were designed from the 16S-23S rRNA intergenic spacer region of N. salmonicida, and conditions for LAMP were optimized as incubating all the reagents for 60 min at 64 degrees C. LAMP products were judged with agar gel electrophoresis as well as with the naked eye after the addition of SYBR Green I. Results showed the sensitivity of the LAMP assay was 1.68 x 10(3) CFU/ml (16.8 CFU per reaction) and 10-fold higher than that of PCR. The LAMP method was also effectively applied to detect N. salmonicida in diseased fish samples, and it may potentially facilitate the surveillance and early diagnosis of fish nocardiosis.