文献类型：期刊文献

英文题名：Anticoagulant and anti-inflammatory effects of a degraded sulfate glycosaminoglycan from swimming bladder

作者：Chen, Jing[1];Zhou, Siyi[1,2];Wang, Zhuo[1];Liu, Shouchun[1];Li, Rui[1];Jia, Xuejing[1];Chen, Jianping[1];Liu, Xiaofei[1];Song, Bingbing[1];Zhong, Saiyi[1,3,4]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Engn Technol Res Ctr Seafood, Guangdong Prov Key Lab Aquat Prod Proc & Safety,Gu, Zhanjiang 524088, Peoples R China;[2]Shanwei Inst Technol, Shanwei 516600, Peoples R China;[3]Guangdong Ocean Univ, Shenzhen Res Inst, Shenzhen 518108, Peoples R China;[4]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China

年份：2022

卷号：157

外文期刊名：FOOD RESEARCH INTERNATIONAL

收录：SCI-EXPANDED(收录号：WOS:000822974800010)、、EI(收录号：20222612265843)、Scopus(收录号：2-s2.0-85132441015)、WOS

基金：Acknowledgements This work was supported in part by Key-Area Research and Devel-opment Program of Guangdong Province (2020B1111030004) , National Key Research and Development Project (2019YFD0902005) , the Science and Technology Program of Zhanjiang City (2019A01033) and the Innovative Team Program of High Education of Guangdong Province (2021KCXTD021) .

语种：英文

外文关键词：Anti-inflammatory; Degradation; Hyaluronidase; Low molecular sulfate glycosaminoglycan; Swimming bladder

外文摘要：Low molecular weight sulfate glycosaminoglycan has attracted more attention recently for its great bioactivity. In the present study, a degraded sulfate glycosaminoglycan (named D-SBSG) was prepared from swimming bladder by enzymatic depolymerization, the structure characteristics of D-SBSG and its effects on blood coagulation and inflammation in vitro was investigated. HPGPC analysis showed that the molecular weight (Mw) of SBSG was 115.84 kDa, while the Mw of D-SBSG was 4.96 kDa. The bioactivities had arose dramatic differences, though its main molecule structure had little change after enzymatic degradation. Compared with heparin sodium, relatively milder anticoagulant activity in vitro, which were positively associated with molecular weight, were found in SBSG and D-SBSG. In contrast, the results of anti-inflammatory assays indicated that D-SBSG with the lower molecular weight possessed higher bioactivity than SBSG. Additionally, the D-SBSG inhibited the LPSinduced inflammatory in RAW264.7 macrophages by down-regulation of inflammatory mediators, both of NF-kappa B (including p65) and MAPK (including p38) signaling pathways to exert its anti-inflammatory function. These results indicated that enzymolysis is a viable strategy for degradation of sulfate glycosaminoglycan, and D-SBSG could be a promising ingredient for inflammation management.