文献类型：期刊文献

英文题名：Characterization and identification of streptococci from golden pompano in China

作者：Cai, X. H.[1,2];Peng, Y. H.[2];Wang, Z. C.[2];Huang, T.[3];Xiong, X. Y.[2];Huang, Y. C.[1];Wang, B.[1];Xu, L. W.[4];Wu, Z. H.[1]

机构：[1]Guangdong Ocean Univ, Guangdong Key Lab Control Dis Aquat Econ Anim, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China;[2]Guangxi Inst Oceanol, Guangxi Key Lab Marine Biotechnol, Beihai 536000, Peoples R China;[3]Guangxi Acad Fishery Sci, Guangxi Key Lab Aquat Genet Breeding & Hlth Aquac, Guangxi 530021, Peoples R China;[4]Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China

年份：2016

卷号：119

期号：3

起止页码：207

外文期刊名：DISEASES OF AQUATIC ORGANISMS

收录：SCI-EXPANDED(收录号：WOS:000380278200004)、、Scopus(收录号：2-s2.0-84971427260)、WOS

基金：We thank all laboratory members' suggestions on this manuscript. This work was supported by the National 'Twelfth Five-Year' Plan for Science & Technology Support of China (grant no. 2012BAD17B03), National Natural Science Foundation of China (grant no. 31302226), Science & Technology Planning Project of Guangdong Province of China (grant no. 2013B020307014), Natural Science Foundation of Guangxi Province of China (grant nos. 2015GXNSFAA139088, 2012GXNSFAA053065, 1222013-2, and GXIF-2012-02), and the National Public Welfare Project (grant no. 201205028-4).

语种：英文

外文关键词：Trachinotus ovatus; Streptococcus; Pathogenicity; Biochemical characterization; Multiplex PCR assay

外文摘要：Streptococcal infections cause significant mortality and high economic losses in the fish farm industry worldwide, including in the culture of golden pompano Trachinotus ovatus L., a species gaining popularity in China. A total of 9 streptococcal strains were isolated from cage-cultured diseased golden pompano in Beihai, Zhanjing, and Shenzhen, China, between 2012 and 2014. Conventional and rapid identification systems were used to determine that the isolates were Streptococcus agalactiae, S. iniae, and S. dysgalactiae subsp. dysgalactiae. All isolates were gram-positive cocci cells in pairs or short-chain, non-motile, catalase negative, alpha or beta hemolytic cocci. The results of multiplex PCR assays and 16S rRNA BLAST analysis also showed that the beta hemolytic strains were S. agalactiae and S. iniae and the alpha hemolytic strain was S. dysgalactiae subsp. dysgalactiae, respectively. Pathogenicity assays revealed that S. agalactiae (lethal dose [LD50]: 6.38 x 10(4) CFU ml(-1)) was more virulent for golden pompano than S. iniae (LD50: 1.47 x 10(7) CFU ml(-1)) and S. dysgalactiae subsp. dysgalactiae (LD50: 2.57 x 10(6) CFU ml(-1)) when they were challenged by intraperiotoneal (i.p.) injection. The results of antibiotic susceptibility showed that all strains were extremely susceptible to cefradine, erythromycin, and cefotaxime but resistant to gentamicin, penicillin G, novobiocin, neomycin, ciprofloxacin, roxithromycin, furazolidone, enrofloxacin, norfloxacin, kanamycin, ampicillin, tetracycline, and vancomycin This is the first report of a phenomenon of golden pompano coinfection with S. agalactiae and S. iniae, which will contribute to the diagnosis and prevention of streptococcicosis.