文献类型：期刊文献

英文题名：miR-10a-3p Participates in Nacre Formation in the Pearl Oyster Pinctada fucata martensii by Targeting NPY

作者：Yang, Min[1]; Li, Xin lei[1]; Zhang, Yu ting[1]; Deng, Yue wen[1,2,3,4]; Jiao, Yu[1,2,3]

机构：[1] Fishery College, Guangdong Ocean University, Zhanjiang, 524025, China; [2] Pearl Breeding and Processing Engineering Technology Research Centre of Guangdong Province, Zhanjiang, 524088, China; [3] Guangdong Science and Innovation Center for Pearl Culture, Zhanjiang, 524088, China; [4] Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy culture, Zhanjiang, 524088, China

年份：2023

卷号：25

期号：3

起止页码：428

外文期刊名：Marine Biotechnology

收录：EI(收录号：20232214168251)、Scopus(收录号：2-s2.0-85160452344)

语种：英文

外文关键词：Gems - Gene expression - RNA

外文摘要：MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression via the recognition of their target messenger RNAs. MiR-10a-3p plays an important role in the process of ossification. In this study, we obtained the precursor sequence of miR-10a-3p in the pearl oyster Pinctada fucata martensii (Pm-miR-10a-3p) and verified its sequence by miR-RACE technology, and detected its expression level in the mantle tissues of the pearl oyster P. f. martensii. Pm-nAChRsα and Pm-NPY were identified as the potential target genes of Pm-miR-10a-3p. After the over-expression of Pm-miR-10a-3p, the target genes Pm-nAChRsα and Pm-NPY were downregulated, and the nacre microstructure became disordered. The Pm-miR-10a-3p mimic obviously inhibited the luciferase activity of the 3′ untranslated region of the Pm-NPY gene. When the interaction site was mutated, the inhibitory effect disappeared. Our results suggested that Pm-miR-10a-3p participates in nacre formation in P. f. martensii by targeting Pm-NPY. This study can expand our understanding of the mechanism of biomineralization in pearl oysters. ? 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.