文献类型：期刊文献

英文题名：PLZF-Induced Upregulation of CXCR4 Promotes Dairy Goat Male Germline Stem Cell Proliferation by Targeting Mir146a

作者：Mu, Hailong[1];Li, Na[1];Wu, Jiang[1,2];Zheng, Liming[1];Zhai, Yuanxin[1];Li, Bo[1];Song, Wencong[1];Wang, Jinglu[1];Zhu, Haijing[1,3];Li, Guangpeng[4];Hua, Jinlian[1]

机构：[1]Northwest A&F Univ, Coll Vet Med, Shaanxi Ctr Stem Cells Engn & Technol, Yangling 712100, Shaanxi, Peoples R China;[2]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524088, Guangdong, Peoples R China;[3]Yulin Univ, Coll Life Sci, Yulin 719000, Shaanxi, Peoples R China;[4]Inner Mongolia Univ, Key Lab Mammalian Reprod Biol & Biotechnol, Minist Educ, Hohhot 010021, Peoples R China

年份：2016

卷号：117

期号：4

起止页码：844

外文期刊名：JOURNAL OF CELLULAR BIOCHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000370159900004)、、Scopus(收录号：2-s2.0-84958146253)、WOS

基金：Grant sponsor: Program of National Natural Science Foundation of China; Grant numbers: 31272518, 31572399; Grant sponsor: National Major Project for Production of Transgenic Breeding; Grant number: 2014ZX08007-002; Grant sponsor: National High Technology Research and Development Program of China; Grant number: SS2014AA021605; Grant sponsor: Doctoral Fund of Ministry of Education of China, RFDP; Grant number: 20120204110030; Grant sponsor: National Major Fundamental Research Program of China; Grant number: 2013CB967401.

语种：英文

外文关键词：mir146a; SPERMATOGONIAL STEM CELL (SSC); MALE GERMLINE STEM CELLS (mGSCs); CXCL12-CXCR4; SELF-RENEWAL; DAIRY GOAT

外文摘要：Previous studies have shown that promyelocytic leukemia zinc finger (PLZF), chemokine (C-X-C motif) receptor 4 (CXCR4) and mir146a were associated with the self-renewal of mouse spermatogonial stem cells (SSCs); however, there is little information on their effects on the fate of livestock SSCs. Here, we have identified a regulatory pathway in dairy goat mGSCs, involving PLZF, mir146a and the SDF-1 receptor CXCR4. PLZF overexpression downregulated mir146a and simultaneously upregulated the expression of CXCR4 protein, whereas PLZF knockdown (siPLZF) induced the specifically opposite effects. The in vitro assays demonstrated that PLZF specifically interacts with and suppresses the mir146a promoter, and mir146a targets CXCR4 to impede its translation. The levels of ERK1/2 phosphorylation in the mGSCs overexpressed CXCR4 and PLZF were upregulated, respectively, whereas mir146a expression was decreased and CXCR4 protein was increased. Mir146a overexpression and siPLZF impaired mGSC proliferation and differentiation, however, Mir146a knockdown induced the opposite effects. The effects of PLZF and mir146a were mediated regulation by mir146a and CXCR4, respectively. Overexpression of CXCR4 or addition of CXCL12 in cultures of dairy goat mGSCs resulted in the upregulation of their signaling, and the phosphorylation of ERK1/2 was increased. Collectively, these findings indicate that PLZF is an important transcription factor in the regulation of the expression of CXCR4 to promote dairy goat mGSC proliferation by targeting mir146a. J. Cell. Biochem. 117: 844-852, 2016. (c) 2015 Wiley Periodicals, Inc.