文献类型：期刊文献

英文题名：Partially Acid-Hydrolyzed Porphyran Improved Dextran Sulfate Sodium-Induced Acute Colitis by Modulation of Gut Microbiota and Enhancing the Mucosal Barrier

作者：Yu, Biao[1,2];Wang, Min[2,3];Teng, Bo[2];Veeraperumal, Suresh[2];Cheung, Peter Chi-Keung[4];Zhong, Saiyi[1];Cheong, Kit-Leong[1,2]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Peoples R China;[2]Shantou Univ, Coll Sci, Dept Biol, Shantou 515063, Guangdong, Peoples R China;[3]Guangdong Ocean Univ, Postgrad Coll, Zhanjiang 524088, Peoples R China;[4]Chinese Univ Hong Kong, Sch Life Sci, Food & Nutr Sci Program, Hong Kong 999077, Peoples R China

年份：2023

卷号：71

期号：19

起止页码：7299

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000984422800001)、、EI(收录号：20232114116818)、Scopus(收录号：2-s2.0-85159626326)、WOS

基金：This work was supported in part by Key-Area Research and Development Program of Guangdong Province (2020B1111030004) and The Innovative Team Program of High Education of Guangdong Province (2021KCXTD021) .

语种：英文

外文关键词：Porphyra haitanensis; porphyran; mucus; gut microbiota; ulcerative colitis

外文摘要：The pharmacological values of marine algal polysaccharides on gut health are being recognized in recent research. However, the protective effect of degraded polysaccharides from Porphyra haitanensis (PHP-D) on the colonic mucosal barrier damaged in ulcerative colitis is poorly understood. The purpose of this study was to investigate how PHP-D could maintain the integrity of colonic mucosal layer mediated by microbiota in a dextran sulfate sodium (DSS)-induced colitis mouse model. Structural analysis revealed that PHP-D had a typical porphyran structure having a backbone of alternating (1-* 3)-linked beta-D-galactopyranose units linked to either (1-* 4)-3,6-anhydro-alpha-L-galactopyranose units or (1-* 4)-linked alpha-L-galactose-6-sulfate units. An in vivo study demonstrated that PHP-D treatment reduced the severity of DSS-induced ulcerative colitis. 16S rRNA phylogenetic sequencing revealed that PHP-D affected the diversity of gut microbiota with an increase of Bacteroides, Muribaculum, and Lactobacillus species. Similarly, PHP-D increased levels of short-chain fatty acids. Furthermore, PHP-D restored mucus thickness and improved the expression of tight junction proteins. This work demonstrates that PHP-D is capable of enhancing a colonic mucosal barrier. These outcomes offer unique perspectives on the potential application of P. haitanensis as a promising natural product for the management of ulcerative colitis.