文献类型：期刊文献

英文题名：Isolation and Characterization of a Heparin-Like Compound with Potent Anticoagulant and Fibrinolytic Activity from the Clam Coelomactra antiquata

作者：Du, ZhenXing[1,2,3];Jia, XueJing[1,3];Chen, Jing[1,2,3];Zhou, Siyi[1,2,3];Chen, JianPing[1];Liu, XiaoFei[1];Cao, XiaoHuang[1];Zhong, Saiyi[1,2,3,4];Hong, PengZhi[1,2,3]

机构：[1]Guangdong Ocean Univ, Sch Food Sci & Technol, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518108, Guangdong, Peoples R China;[3]Guangdong Prov Engn Lab Marine Biol Prod, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Peoples R China;[4]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China

年份：2020

卷号：18

期号：1

外文期刊名：MARINE DRUGS

收录：SCI-EXPANDED(收录号：WOS:000513184600003)、、WOS

基金：This work was supported in part by National Key Research and Development Project (2019YFD0902005), the Science and Technology Development Program of Shenzhen City, China (JCYJ20170818111335796), the Science and Technology Program of Zhanjiang City (2019A01015), and Special Funds for the National Natural Science Foundation of China (31972163).

语种：英文

外文关键词：shellfish; heparin; structural characteristics; anticoagulant activity; fibrinolytic activity

外文摘要：Heparin from mollusks with unique sulfated glycosaminoglycan exhibits strong antithrombotic activities. This study reports on a purified heparinoid from Coelomactra antiquata, which shows potent anticoagulant and fibrinolytic abilities. Its structure was characterized by infrared spectroscopy, high-performance liquid chromatography, and one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy. Its fibrinolytic activity was determined in vitro and in vivo. Its anticoagulant activity was determined by activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT). The results indicated that clam heparinoid was a homogeneous glycosaminoglycan with a molecular weight of 30.99 kDa, mainly composed of -> 4)-alpha-IdoA2S-(1 >4)-alpha-G1cNS3S6S (or GlcNS6S)-(1 >4)-(3-GlcA-(1 >4)-alpha--G1cNS6S (or GlcNAC)-(1 >. Furthermore, this heparinoid showed a highly anticoagulant titer and fibrinolytic value of 149.63 IU/mg and 1.96 IU/mg, respectively. In summary, clam heparinoid shows great potential for application in the clinic and antithrombotic drugs industry.