文献类型：期刊文献

英文题名：Response mechanism of ♀ Epinephelus fuscoguttatus x ♂ Epinephelus lanceolatus under low-temperature and waterless stresses using TMT proteomic analysis

作者：Fan, Xiuping[1,2];Guo, Qiaoyu[1];Zhang, Jiasheng[1];Du, Huan[1];Qin, Xiaoming[1,2]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol,Key Lab Adv Proc Aquat Pr, Guangdong Prov Engn Technol Res Ctr Marine Food, Guangdong Prov Key Lab Aquat Product Proc & Safet, Zhanjiang 524088, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab, Zhanjiang 524088, Peoples R China

年份：2022

卷号：259

期号：1

起止页码：217

外文期刊名：PROTOPLASMA

收录：SCI-EXPANDED(收录号：WOS:000647527900001)、、WOS

基金：This study was supported by National Key R&D Program of China (grant number 2019YFD0901601), Southern Marine Science and Engineering Guangdong Laboratory (grant number ZJW-2019-06), and Guangdong University Innovation Entrepreneurship Training Project (grant number CXXL2019156).

语种：英文

外文关键词：Response mechanism; ♀ Epinephelus fuscoguttatus; ♂ Epinephelus lanceolatus; Low-temperature; Waterless stresses; TMT proteomic analysis

外文摘要：female Epinephelus fuscoguttatus x male Epinephelus lanceolatus, a hybrid grouper created from artificial breeding, has been widely developed over the past decades. However, the study focusing on lukewarm high-protein-content fish species using advanced techniques has rarely been reported. In this work, the TMT (tandem mass tag)-assisted technique was employed to explore its differentially expressed proteins and response mechanisms under low-temperature dormant and waterless stresses. Our findings suggest that 162 and 258 differentially expressed proteins were identified under low-temperature dormant and waterless stresses, respectively. The waterless preservation treatment further identifies 93 differentially expressed proteins. The identified proteins are categorized and found to participate in lipid metabolism, glycometabolism, oxidative stress, immune response, protein and amino acid metabolism, signal transduction, and other functions. Accordingly, the factors that affect the response mechanisms are highlighted to provide new evidences at protein level.