文献类型：期刊文献

英文题名：Identification, characterization, and expressions profile analysis of growth hormone receptors (GHR1 and GHR2) in Hybrid grouper (Epinephelus fuscoguttatus female x Epinephelus polyphekadion male)

作者：Amenyogbe, Eric[1,2];Chen, Gang[1,2];Wang, Zhongliang[1,2]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524025, Guangdong, Peoples R China;[2]Guangdong Higher Educ Inst, Lab Fish Aquaculture, Guangdong Prov Key Lab Aquaculture South China Se, Zhanjiang 524025, Peoples R China

年份：2020

卷号：112

期号：1

起止页码：1

外文期刊名：GENOMICS

收录：SCI-EXPANDED(收录号：WOS:000506628500001)、、WOS

基金：We acknowledge China Agriculture research system (CARS-47) as well as Professor Chen Gang and all teachers under him for their patience and support all through this research work.

语种：英文

外文关键词：Growth hormone receptor; Real-time PCR; Tissue expression; Bioinformatics; Hybrid grouper

外文摘要：Growth hormone is an essential hormone that plays essential roles in growth, metabolism, cellular differentiation, immunity and reproduction in fish, by means of the growth hormone receptors. The encoding cDNA growth hormone receptors (GHR1 and GHR2) were cloned and characterized from Hybrid grouper (Epinephelus fuscoguttatus female x Epinephelus polyphekadion male). Sequence analysis of the cloned GHR1 was observed as containing 2176, which comprised an ORF of 1842 bp, 5 UTR of 6 bp and 3 UTR of 328 bp, with 612 amino acids encoding proteins, while GHR2 was observed as containing 1824 bp that encompassed an ORF of 708 bp, 5 UTR of 48 bp and 3 UTR of 1068 bp with 235 amino acids encoding proteins. Relative mRNA expression of GHR1 and GHR2 in the liver and muscle was found to be highest respectively. Our findings provide vital statistics of GHRs likely to play a significant role in the growth of the fish.