文献类型：期刊文献

中文题名：一株拮抗海鱼干优势耐盐真菌芽孢杆菌HY-5筛选及其抗菌成分鉴定

英文题名：Identification of Antagonistic Bacillus HY-5 and Its Antimicrobial Compositions from Mangrove Against Specific Salt Resistance Fungi of Marine Dried Fish

作者：杨秀文[1];邓旗[1];陶森[1];孙力军[1];王雅玲[1];廖建萌[2];刘颖[1];房志家[1];徐德峰[1]

机构：[1]广东海洋大学食品科技学院广东省水产品加工与安全重点实验室,广东湛江524088;[2]湛江市食品药品检验所,广东湛江524000

年份：2018

卷号：39

期号：16

起止页码：120

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD、、CSCD_E2017_2018、北大核心2017、北大核心、CSCD

基金：广东省科技厅公益研究与能力建设专项资金项目(2014B020204005)

语种：中文

中文关键词：海洋源解淀粉芽孢杆菌;真菌;海鱼干;脂肽;液相色谱-质谱法(LC-MS)

外文关键词：marine Bacillus amyloliquefacien;fungi;marine dry fish;lipopeptide;liquid chromatography - mass spectrometry（LC-MS）

中文摘要：目的:开发新的海鱼干耐高盐优势真菌抑制剂。方法:取用湛江市特呈岛红树林海域的海水、海泥以及红树林的根、茎和叶的样本,利用琼脂扩散法和平板点种法筛选出一株强抗菌活性菌株HY-5,通过传统细胞形态观察并结合生理生化实验及16S r DNA序列进行分析鉴定;利用杯碟法测定上清液对17株霉变海鱼干优势真菌抗菌谱,发酵液酸沉后经LC-MS检测其抗菌成分。结果:HY-5被鉴定为解淀粉芽孢杆菌,其上清液对17株霉变海鱼干优势真菌均有明显抗菌作用;LC-MS检测到HY-5发酵液中含2组抗菌脂肽surfactin(994.6、1008.6、1022.5和1036.6)和iturin(1016.5、1030.5、1044.4和1058.3)组分。结论:海洋源解淀粉芽孢杆菌HY-5的粗提液中含有抗菌脂肽,其对霉变海鱼干优势真菌有较强的拮抗作用。

外文摘要：Objective： To discover a new antagonistic bacillus aganist specific fungi possessed high salt resistance of marine dry fish.Method： A strain with high antimicrobial activity named HY-5 was screened from seawater,sea mud,leaves and bark from mangroves of Zhanjiang by agar diffusion method and plate method. HY-5 was identified as Bacillus amyloliquefaciens by traditional cell morphology,physiological,biochemical experiments and 16 S r DNA sequence analysis.The supernatant of HY-5 fermentation with obvious antibacterial effect on 17 strains fungi which isolated from moldy marine dried fish was determined by cup-dish method.And the fermentation broth contained two groups of antimicrobial lipopeptide surfactin（ 994.6,1008.6,1022.5 and 1036.6） and iturin（ 1016.5,1030.5,1044.4 and 1058.3） by LC-MS method. Conclusion： The crude extract of Bacillus amyloliquefaciens HY-5 contained antimicrobial lipopeptides which had strong antagonistic effect on fungi isolated from the marine dry fish.