文献类型：期刊文献

中文题名：猪前脂肪细胞分化过程中RETN基因的表达

英文题名：Study on RETN gene expression during the differentiation of porcine preadipocyte in vitro

作者：毋文静[1];刘铀[1];张平平[1];李勇[1];刘艳芬[1]

机构：[1]广东海洋大学农学院,广东湛江524088

年份：2011

卷号：41

期号：10

起止页码：1052

中文期刊名：中国兽医科学

外文期刊名：Chinese Veterinary Science

收录：CSTPCD、、CSCD2011_2012、北大核心2008、北大核心、CSCD

语种：中文

中文关键词：抵抗素基因;前脂肪细胞;分化;猪

外文关键词：resistin gene（RETN）; preadipocyte; differentiation; porcine

中文摘要：为探讨猪前脂肪细胞分化过程中抵抗素基因(RETN)表达水平的变化规律,从1日龄仔猪皮下脂肪组织分离前脂肪细胞,于DMEM/F12培养基中培养3、5、7、10d后收获细胞。通过油红O染色法观察前脂肪细胞分化,并用实时荧光定量RT-PCR检测前脂肪细胞分化过程中RETN基因表达水平的变化。结果,在前脂肪细胞分化过程中,RETN基因表达水平显著下降(P<0.01),细胞形态亦发生相应变化,即细胞变圆,细胞内脂肪滴体积增大。结果表明,RETN基因与前脂肪细胞脂肪代谢调节有关,其表达水平下调能促进前脂肪细胞分化。

外文摘要：The aim of this study was to investigate the resistin gene（RETN） expression during the differentiation of porcine preadipocyte in vitro.The preadipocytes were isolated from subcutaneous fat tissue of one-day-old piglet and cultured in the DMEM/F12 media for 3,5,7 and 10 days respectively.The cell differentiation was evaluated by intracellular lipid droplet stained by red oil O,and the RETN gene expression during the preadipocyte differentiation were determined by real-time fluorescence quantitative RT-PCR simultaneously.In result,during the preadipocyte differentiation,the level of RETN expression was significantly decreased（P0.01）,and evident changes of the preadipocyte,including rounded fat cells in morphology and increased volume of intracellular lipid droplets,were found accordingly.In conclusion,RETN gene may be associated with fat metabolism regulation,and the differentiation of porcine preadipocyte can be promoted by down-regulation of RETN gene expression.