文献类型：期刊文献

英文题名：Effect of protein sources in formulated diets on the growth, immune response, and intestinal microflora of pearl oyster Pinctada fucata martensii

作者：Liao, Yongshan[1];Cai, Caixia[1];Yang, Chuangye[1];Zheng, Zhe[1,2];Wang, Qingheng[1,2];Du, Xiaodong[1,2];Deng, Yuewen[1,2]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Pearl Breeding & Proc Engn Technol Res Ctr Guangd, Zhanjiang 524088, Peoples R China

年份：2020

卷号：16

外文期刊名：AQUACULTURE REPORTS

收录：SCI-EXPANDED(收录号：WOS:000517752000019)、、WOS

基金：This work was supported by the Science and Technology Department of Guangdong Province (grant number 2014A020208122 and 2019A1515011096); "Innovation Team Project" (grant number 2017KCXTD016) from the Department of Education of Guangdong Province; the Graduate education innovation program of Guangdong Ocean University (grant number 201835) and the China Agriculture Research System (grant number CARS-049).

语种：英文

外文关键词：Pinctada fucata martensii; Formulated diet; Intestinal microflora; 16S rRNA sequencing

外文摘要：Diets affect the intestinal microflora, which in turn affects the growth performance and immune response on host. To study the effect of protein sources in formulated diets on the growth, immune response, and intestinal microflora of pearl oyster Pinctada fucata martensii, two major protein sources (yeast powder-D1 and corn gluten-D2) were used in the preparation of two isonitrogenous and isolipidic diets. The pearl oysters were divided into two groups, namely, ED1 and ED2, which were fed with D1 and D2 indoors respectively. After 45-day culturing, ED1 had significantly higher growth performance and protease and amylase activities than ED2. ED1 also had significantly higher alkaline phosphatase, catalase, superoxide dismutase, glutathione peroxidase activities and total antioxidant capacity. A 16S rRNA-based sequencing revealed that the main bacterial phyla in ED1 were Proteobacteria, Firmicutes, Tenericutes, and Bacteroidetes, which accounted for 97.70 % of the microbial population. Firmicutes were the main phylum in ED2 and accounted for 97.98 %. ED1 got significantly higher Sobs, Chao, ACE, and Shannon indices than ED2. ED1 achieved significantly lower Simpson index than ED2. Results suggested that diet with yeast powder as the major protein source promoted the growth, digestive activity, immune- and antioxidant activity, and intestinal bacterial diversity in pearl oysters. This study provides basic data with regard to the mechanism through which formulated diets enhance digestive tract health of shellfish under controlled conditions.