文献类型：期刊文献

英文题名：Enhanced microbial degradation of pentachlorophenol from soil in the presence of earthworms: Evidence of functional bacteria using DNA-stable isotope probing

作者：Li, Xiaomin[1];Lin, Zhong[1,2];Luo, Chunling[3];Bai, Jing[1];Sun, Yingtao[1];Li, Yongtao[1]

机构：[1]South China Agr Univ, Coll Nat Resources & Environm, Guangzhou 510642, Guangdong, Peoples R China;[2]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524088, Peoples R China;[3]Chinese Acad Sci, Guangzhou Inst Geochem, Guangzhou 510640, Guangdong, Peoples R China

年份：2015

卷号：81

起止页码：168

外文期刊名：SOIL BIOLOGY & BIOCHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000350524700020)、、EI(收录号：20150100393276)、Scopus(收录号：2-s2.0-84949114989)、WOS

基金：This work was financially supported by the National Nature Science Foundation of China (Project Nos. 41171210 and 41301252).

语种：英文

外文关键词：Earthworm; Pentachlorophenol degradation; Functional bacteria; DNA stable isotope probing

外文摘要：This study investigated the effect of two earthworm species (Amynthas robustus Perrier and Eisenia fetida Savigny) on the soil microbial degradation of pentachlorophenol (PCP). PCP-degrading microbes were identified using DNA-stable isotope probing (SIP). The results showed that adsorption and fixing to soil particles and organic fractions dominated the fate of PCP in soil without any amendments. The inoculation of both earthworm species significantly enhanced soil PCP disappearance and basal respiration. The DNA-SIP results revealed that Klebsiella, Cupriavidus, Aeromonas, and Burkholderia spp. were present at higher relative abundances in [C-13]abeled-PCP-amended soil microcosms than [C-12]-PCP-amended soil in the presence of A. robustus, indicating that these bacterial species were responsible for PCP assimilation. Cupriavidus and Aeromonas sp. were also detected in the earthworm gut before inoculation, and their relative abundance was affected by earthworms. These results demonstrated that earthworms can introduce functional bacteria into soils and increase the population of PCP-degrading bacteria, thereby accelerating soil PCP degradation. (C) 2014 Elsevier Ltd. All rights reserved.