文献类型：期刊文献

中文题名：酪蛋白双酶复合酶解与混合酶解作用研究

英文题名：Study of Effect Double Enzyme Complex Enzymatic Hydrolysis and Mixed Enzymatic Hydrolysis of Casein

作者：方富永[1];苗艳丽[2];陈绍红[1];林倩雯[2];黎敏[2];宋文东[2]

机构：[1]广东海洋大学生物化学中心广东湛江524088;[2]广东海洋大学理学院广东湛江524088

年份：2013

卷号：13

期号：5

起止页码：111

中文期刊名：中国食品学报

外文期刊名：Journal of Chinese Institute Of Food Science and Technology

收录：CSTPCD、、北大核心2011、EI(收录号：20132816496980)、Scopus(收录号：2-s2.0-84879856056)、北大核心、CSCD、CSCD2013_2014

基金：广东省省部产学研结合项目(No.2011B090400252);国家海洋局海洋公益性行业科研专项经费项目(No.200905021);广东省社会发展领域科技计划项目(No.93043)

语种：中文

中文关键词：复合酶解;混合酶解;水解度

外文关键词：composite enzymolysis; mixed enzymatic; hydrolysis degree

中文摘要：目的:研究木瓜蛋白酶与菠萝蛋白酶复合酶解、混合酶解酪蛋白的作用,比较不同水解方式的作用效果。方法:分别进行两种蛋白酶单因素水解试验,寻找各种酶的最适水解条件;在此基础上进行复合酶解与混合酶解试验,测定水解度。结果:复合酶解水解度与加酶顺序有关,也与加新酶前是否灭活旧酶有关,对于相同组合,灭活酶的水解度比不灭活酶的低。混合酶解的水解度与水解时间有关,最适水解时间与单酶最适水解时间最长的相当;混合酶解水解度高于复合酶解。结论:本试验条件下,不灭活酶组合水解度比灭活酶的高,混合酶解水解度比复合酶解的高。

外文摘要：Objective：Research on casein affected by the systems about mixed enzyme and composite enzyme including papain and pineapple, comparing with the result in different ways of hydrolysis. Method：First, the experiment went on with the single factor of two proteases hydrolyzing casein to explore the optimum condition of enzymatic hydrolysis. On this basis, started the experiment on composite enzymatic hydrolysis and mixed enzyme to determinate the degree of hydrolysis. Consequence： The degree of hydrolysis about composite enzyme relates to the sequence of adding enzyme and also be affected whether inactivating the old enzyme before adding the new one. To the same combination, the degree of hydrolysis with inactivated enzyme is lower than the non-inactivated one. Mixed enzymatic hydrolysis degree and hydrolysis time length has a direct relationship, hydrolysis time and the longest single enzyme hydrolysis optimum time equivalent; mixed enzymatic hydrolysis degree higher than by composite enzymatic hydrolysis. Conclusion： In the experimental conditions, no inactivation of enzyme combination hydrolysis degree higher than inactivated enzyme combination, mixed enzymatic hydrolysis degree higher than by composite enzymatic hydrolysis.