文献类型：期刊文献

英文题名：Comparison of bacterioplankton communities in three mariculture ponds farming different commercial animals in subtropical Chinese coast

作者：Wei, Chaoling[2];Zeng, Yonghui[3];Tang, Kunxian[1];Jiao, Nianzhi[1]

机构：[1]Xiamen Univ, State Key Lab Marine Environm Sci, Xiamen 361005, Peoples R China;[2]Anhui Agr Univ, Sch Resources & Environm, Hefei 230036, Anhui, Peoples R China;[3]Guangdong Ocean Univ, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524025, Guangdong, Peoples R China

年份：2009

卷号：632

期号：1

起止页码：107

外文期刊名：HYDROBIOLOGIA

收录：SCI-EXPANDED(收录号：WOS:000268728500006)、、EI(收录号：20094312396714)、Scopus(收录号：2-s2.0-70350011943)、WOS

基金：This study was supported by the NSFC projects ( 40632013, 40521003), and the MOST project ( 200805068, 2006BAC11B04). We are grateful to Yanlin Zhao and Dechao Yang for sampling and field assistance. We thank Dr. Oliver Yu at the Donald Danforth Plant Science Center, USA, for his help in editing and improving the manuscript. Thanks are also due to the editor for his detailed revisions on the language and contents, and to the reviewers for their critical comments.

语种：英文

外文关键词：Bacterial community composition; Free-living; Diversity; 16S rDNA; Clone library; Mariculture pond; Coastal environment

外文摘要：In order to explore the responses of the bacterioplankton community to different types of aquaculture environments, three mariculture ponds comprised of groupers (Epinephelus diacanthus, ED), prawns (Penaeus vannamei, PV), and abalone (Haliotis diversicolor supertexta, HDS) in southeast, coastal China were investigated. The free-living bacterial diversity was analyzed through the construction of 16S rDNA clone library. A total of 203 16S rDNA sequences from three clone libraries were classified into 118 operational taxonomic units (OTUs), of which 51, 31, and 42 OTUs were distributed in the ED, PV, and HDS pond, respectively, with Bacteroidetes (30.6%), Actinobacteria (55.2%), and Cyanobacteria (32.8%) as the dominant division in the respective ponds. Meanwhile, each pond occupied some unique OTUs that were affiliated with uncommon (sub-) phyla, such as candidate OP11 division, Acidobacteria, Deltaproteobacteria, Planctomycetes, and Verrucomicrobia. Bacterial diversity in the ED pond was the richest, followed by the HDS and the PV pond. OTUs of 61.9% and 94.9% have less than 90% and 97% similarity to their nearest neighbors in public databases, respectively. All OTUs were grouped into 67 clusters, covering 11 (sub-) phyla. The OTUs only from single pond distributed in 53 clusters (79.1%), the OTUs shared by two ponds were affiliated with 14 clusters (20.9%), and none of clusters was formed by the OTUs which commonly originated from the three pond libraries, suggesting that the composition of bacterial populations in these ponds were significantly different. These results indicate that the aquatic environment created by different mariculture animals may foster very special and complex bacterial communities.