文献类型：期刊文献

中文题名：高糖饲料中补充二甲双胍对珍珠龙胆石斑鱼生长性能、糖酵解酶活性和肠道健康的影响

英文题名：Effects of Metformin Supplementation in High-Carbohydrate Diets on Growth Performance,Glycolytic Enzyme Activities and Intestinal Flora of Hybrid Grouper(Epinephelus fuscoguttatus♀×Epinephelus lanceolatu♂)

作者：于沁彤[1,2,3];张依雯[1,2,3];谭北平[1,2,3];董晓慧[1,2,3];迟淑艳[1,2,3];杨奇慧[1,2,3];章双[1,2,3];温家声[1,2,3];刘泓宇[1,2,3]

机构：[1]广东海洋大学水产学院水产动物营养与饲料实验室,广东湛江524088;[2]农业农村部华南水产与畜禽饲料重点实验室,广东湛江524088;[3]广东省水产动物精准营养与高效饲料工程技术研究中心,广东湛江524088

年份：2025

卷号：46

期号：5

起止页码：85

中文期刊名：渔业科学进展

外文期刊名：Progress in Fishery Sciences

收录：北大核心2023、、北大核心

基金：广东省普通高校重点研究项目(2024ZDZX2085);深圳市自然科学基金(JCYJ20240813111700002);广东省现代海洋牧场适养品种核心技术攻关项目(2024-MRB-00-00-001);广东海洋大学创新项目(CXXL2022008)共同资助。

语种：中文

中文关键词：珍珠龙胆石斑鱼;二甲双胍;糖酵解;肠道菌群

外文关键词：Hybrid grouper;Metformin;Glycolysis;Intestinal microflora

中文摘要：本研究以肉食性的珍珠龙胆石斑鱼(Epinephelus fuscoguttatus♀×E.lanceolatus♂)为研究对象,研究高糖饲料中添加二甲双胍对其生长性能、糖酵解酶活性和肠道菌群的影响。将540尾珍珠龙胆石斑鱼随机分成6组,每组3个重复,每个重复30尾鱼,均重为(10.4±0.01)g。以2种不同糖水平的正对照(20%,PC)和负对照(30%,T0)以及在负对照饲料的基础上分别添加0.2%(T2)、0.4%(T4)、0.6%(T6)和0.8%(T8)的二甲双胍作为实验饲料饲养8周。与对照组相比,高糖饲料显著提高了珍珠龙胆石斑鱼的肝体比(P<0.05),而补充二甲双胍后,脏体比和肝体比显著降低(P<0.05)。饲喂高糖饲料使珍珠龙胆石斑血清中葡萄糖、低密度脂蛋白和甘油三酯含量升高,肝脏葡萄糖-6-磷酸酶(G6Pase)和果糖-1,6-二磷酸酶(FBPase)活性增加(P<0.05),在补充二甲双胍后,葡萄糖、低密度脂蛋白和甘油三酯含量下降,肝脏G6Pase和FBPase活性显著降低,并且葡萄糖激酶(GK)、6-磷酸果糖激酶(PFK-1)和丙酮酸激酶(PK)活性显著增强(P<0.05)。高糖饲料增加了变形菌门(Proteobacteria)、发光杆菌属(Photobacterium)的相对丰度(P>0.05),在高糖饲料中补充二甲双胍后,肠道中厚壁菌门(Firmicutes)、短芽孢杆菌属(Brevibacillus)相对丰度增加,而发光菌属的相对丰度降低。在本实验条件下,当高糖饲料中补充0.2%二甲双胍改变肠道微生物的组成,改善高碳水化合物饲料导致石斑鱼肠道菌群葡萄糖代谢紊乱,从而促进糖酵解和抑制糖异生,进而调节了血糖稳态,有利于提高珍珠龙胆石斑鱼对碳水化合物的利用率。

外文摘要：Hybrid groupers have emerged as a significant aquaculture species owing to their high market value and growing demand.However,carbohydrate-rich diets pose a challenge for efficient glucose metabolism in this species.This phenomenon stems from the limited capacity of fish to effectively metabolize glucose in response to high-carbohydrate diets,resulting in persistent hyperglycemia that adversely affects growth performance.Impaired insulin sensitivity and dysfunction in insulin signaling,characterized by compromised insulin receptor function,blocked insulin cascades,and failure to activate critical signaling pathways,such as the PI3K/AKT pathway,have been identified as key factors contributing to this metabolic disorder.Therefore,this study investigated the application of metformin,a biguanide with recognized hypoglycemic properties,to ameliorate the adverse effects of high-carbohydrate diets in hybrid groupers(Epinephelus lanceolatus×E.fuscoguttatus).A total of 540 pearl gentian grouper,averaging(10.44±0.01)g,were selected and randomly distributed into six treatment groups,each consisting of three replicates with 30 fish per replicate.Six iso-nitrogenous and iso-lipid diets were formulated:A positive control(20%carbohydrate,PC),one negative control(30%carbohydrate,T0),and four experimental groups supplemented with metformin at concentrations of 0.2%(T2),0.4%(T4),0.6%(T6),and 0.8%(T8)relative to the negative control.The results show that the carbohydrate and metformin levels in the feed had no statistically significant effect(P>0.05)on the feed coefficient ratio(FCR)and survival rate(SR).Notably,a high-carbohydrate diet reduced growth performance(WGR,SGR)in hybrid groupers and altered serum biochemical parameters,increasing glucose,triglycerides,and LDL.Meanwhile,decreasing insulin and HDL.Metformin supplementation mitigated these effects,improving lipid metabolism markers and partially restoring HDL levels,with optimal outcomes at higher doses.Growth and organ indices demonstrated linear or quadratic trends in response to metformin,though condition factor(CF)and alpha diversity of gut microbiota were unaffected.Metformin also modulated gut microbial composition,decreasing Proteobacteria and Photobacterium while increasing Firmicutes and Brevibacillus.In the present study,a high-carbohydrate diet inhibited growth performance,decreased insulin sensitivity,and increased the abundance of pathogenic intestinal bacteria in hybrid groupers.In the present study,the administration of a high-sugar diet augmented with 0.2%metformin altered the composition of intestinal microorganisms,thereby enhancing the disruption of glucose metabolism in the intestinal flora of groupers.This disruption is induced by a high-carbohydrate diet that promotes glycolysis and inhibits gluconeogenesis,thereby regulating glucose homeostasis.This,in turn,facilitates an improvement in the utilization rate of carbohydrates by the pearl gentian grouper.