文献类型：期刊文献

英文题名：CHARACTERIZATION OF PATHOGENIC AEROMONAS VERONII BV. VERONII ASSOCIATED WITH ULCERATIVE SYNDROME FROM CHINESE LONGSNOUT CATFISH (LEIOCASSIS LONGIROSTRIS GUNTHER)

作者：Cai, Shuang-Hu[1,2,3];Wu, Zao-He[2,3,4];Jian, Ji-Chang[1,2,3];Lu, Yi-Shan[1,2,3];Tang, Ju-Feng[1,2,3]

机构：[1]Guangdong Ocean Univ, Fishereis Coll, Zhanjiang City, Guangdong, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China;[3]Key Lab Control Dis Aquat Econ Anim Guangdong Hig, Zhanjiang, Peoples R China;[4]Zhongkai Univ Agr & Engn, Guangzhou, Guangdong, Peoples R China

年份：2012

卷号：43

期号：1

起止页码：382

外文期刊名：BRAZILIAN JOURNAL OF MICROBIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000303939800046)、、WOS

基金：This study was supported by the Natural Science Fund of Guangdong Province (No. 94524088010024393). The authors thank all of the relevant fellows who have dedicated time to these experiments.

语种：英文

外文关键词：Aeromonas veronii bv. veronii; ulcerative syndrome; Leiocassis longirostris Gunther; Identification

外文摘要：273 bacterial strains were isolated from 20 Chinese longsnout catfish samples. The biochemical characteristics of all strains conformed to the species description of Aeromonas veronii bv. veronii on the basis of Vitek GNI+ card. Furthermore, 16S rDNA, gyrB and rpoD sequences of the representative strain PY50 were sequenced and showed high similarity with A. veronii bv. veronii in Genbank. Antibiotic-resistance of the representative strain PY50 was assessed by the Kirby-Bauer disk diffusion method, and the results showed it was susceptible and moderately susceptible to 13 and 4 of the 21 antimicrobial agents tested. Extracellular products of strain PY50 contained gelatinase, lecithinase, elastase, most of lipase and lipopolysaccharide. Virulence of strain PY50 and extracellular products to Chinese longsnout catfish were also tested, and LD50 were about 3.47x10(4) CFU per fish and 11.22 mu g per fish in intraperitoneal injection respectively. This is the first report that A. veronii bv. veronii was the pathogenic agent of ulcerative syndrome in Chinese longsnout catfish.