文献类型：期刊文献

英文题名：Metabolomic Profiling identifies Biomarkers of Sexual Dimorphism in the Brain and Gonads of Matured Eleutheronema tetradactylum

作者：Mkulo, Evodia Moses[1];Iddrisu, Lukman[1];Amoah, Kwaku[1,2];Pan, Yifei[1];Miao, Benxun[1];Huang, Jiansheng[1];Wang, Bei[1,2];Li, Jing[1];Wang, Zhongliang[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[2]Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China;[3]Guangdong Prov Marine Fish Technol Innovat Ctr, Zhanjiang 524088, Peoples R China

年份：2026

卷号：28

期号：5

外文期刊名：MARINE BIOTECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:001892120400001)、、EI(收录号：20264021606702)、Scopus(收录号：2-s2.0-105052381863)、WOS

基金：The research leading to these results was financially supported by the 2025 Research on breeding technology of candidate species for Guangdong modern marine ranching (2025-MRB-00-001), the Guangdong Province Ordinary Colleges and Universities Key Field Special Project (Science and Technology Services for Rural Revitalization) (2023ZDZX4011).

语种：英文

外文关键词：Eleutheronema tetradactylum; Protandry; Metabolomics; Neural-gonadal axis; Sex differentiation

外文摘要：Elucidating the metabolic basis of sexual dimorphism is critical for the effective management of sequential hermaphrodites in aquaculture. The four-finger threadfin (Eleutheronema tetradactylum) is a commercially significant protandrous species; however, the metabolic landscapes characterizing its sexual maturity remain unexplored. In this study, we established a foundational metabolomic baseline by profiling brain and gonad tissues from sexually mature males and females using UHPLC-Q-TOF-MS. Multivariate statistical analysis revealed distinct tissue-specific dimorphism, with gonadal tissues exhibiting significantly greater sex-specific metabolic divergence than neural tissues. We identified sex-specific alterations in key metabolic networks, including aminoacyl-tRNA biosynthesis, nucleotide metabolism (purine/pyrimidine), and the glutamine-glutamate axis, which collectively support the high biosynthetic demands of ovarian development. Notably, the brain exhibited subtle yet specific shifts in neuro-lipid signaling, including arachidonic acid metabolism and tryptophan pathways, suggesting a neuroendocrine regulatory role rather than global metabolic remodeling. To our knowledge, this is the first tissue-resolved metabolomic dataset for a protandrous hermaphrodite fish. This study establishes a preliminary metabolomic baseline of biochemical differentiation in E. tetradactylum, identifying candidate biomarkers for sex identification and providing a foundation for future investigation into the metabolic regulation of protandrous hermaphroditism.