文献类型：期刊文献

英文题名：Treatment of Glycine max seeds with gibberellins alters root morphology, anatomy, and transcriptional networks

作者：Han, Y. Q.[1,2];Shi, Y.[3];Gao, Y. M.[1];Du, J. D.[4];Feng, N. J.[4];Zhang, Y. X.[4];Zheng, D. F.[2,5]

机构：[1]Heilongjiang Bayi Agr Univ, Coll Life Sci & Technol, Daqing 163319, Heilongjiang, Peoples R China;[2]Natl Coarse Cereals Engn Res Ctr, Daqing 163319, Heilongjiang, Peoples R China;[3]Fu Dekang Econ & Trade Dev Co, Beijing 100031, Peoples R China;[4]Heilongjiang Bayi Agr Univ, Coll Agr, Daqing 163319, Heilongjiang, Peoples R China;[5]Guangdong Ocean Univ, Coll Agr, Zhanjiang 524088, Guangdong, Peoples R China

年份：2020

卷号：64

起止页码：32

外文期刊名：BIOLOGIA PLANTARUM

收录：SCI-EXPANDED(收录号：WOS:000507993900004)、、WOS

基金：We thank Prof. Robert Aiken of Northwest Research-Extension Center-Colby, Kansas State University for language improvement and valuable suggestions. The authors also thank Prof. A.H. Rajasab of Tumkur University for language improvement. This work was supported by the Institution National Natural Science Foundation of China, China, Project No. 31271652, and the Outstanding Youth Foundation of Heilongjiang Province, China, Project No. JC201309, the Training Project of Heilongjiang Bayi Agricultural University, China, Project No. XZR2015-12, the StartupFoundation for Doctors of Heilongjiang Bayi Agricultural University, Project No. XDB2014-14, XDB201817. The first two authors equally contributed to this work.

语种：英文

外文关键词：gene expression; lateral root; main root; RNA-seq; seed soaking; soybean

外文摘要：Gibberellins (GAs) regulate diverse aspects of growth and development, but their role in root development and lateral root (LR) formation is poorly understood. In this study, GA3 was applied to soybean [Glycine max (L.) Merr] by seed soaking. The results showed that root length and root surface area were significantly inhibited in early stages after GA3 treatment. Microscopic examination showed that GA3 treatment changed the cortex thickness, the pericycle diameter, and cell size in main root. Interestingly, exogenous GA3 increased the quantity of lateral root primordia (LRP), but LR number decreased in this period. Moreover, the content of GAs, auxin and abscisic acid in root was altered. RNA-seq results revealed that application of GA3 not only changed the expression of genes in GA biosynthesis pathway, including GA20ox and GA2ox, but also the GA regulation genes and signalling pathway genes. The changes in expression of gene concerning other hormones were also detected. In addition, GA3 altered cell wall biogenesis and degradation genes which might be related to the changes of root morphology. In response to increased GA3, 103 transcription factors were detected. Thus, exogenous GA3 changed the content of hormones in roots and affected the root development by regulating the expression of respective genes.