文献类型：期刊文献

英文题名：Norethisterone exposure alters the transcriptome of Marine Medaka (Oryzias melastigma) larvae

作者：Li, Xueyou[1];Lin, Xiaona[1];Chen, Yuebi[1];Wang, Zhongduo[1,2,3];Guo, Yusong[1];Afriyie, Gyamfua[1];Zhang, Ning[1];Dong, Zhongdian[1,2]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Guangdong Higher Educ Inst, Key Lab Aquaculture South China Sea Aquat Econ A, Zhanjiang, Peoples R China;[2]Guangdong Ocean Univ, Coll Fisheries, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China;[3]Hunan Normal Univ Sch, Coll life Sci, State Lab Dev Biol Freshwater Fish, Changsha, Peoples R China

年份：2021

卷号：37

期号：9-10

起止页码：767

外文期刊名：CHEMISTRY AND ECOLOGY

收录：SCI-EXPANDED(收录号：WOS:000698213700001)、、Scopus(收录号：2-s2.0-85115231954)、WOS

基金：This work was supported by the National Natural Science Foundation of China [grant numbers 41806195, 31972794]; Special innovation projects of colleges and universities in Guangdong province [grant numbers 2018KTSCX087, 2018KQNCX110]; Nanhai Scholar Project of GDOU [grant numbers QNXZ201807, 201903]; and State Laboratory of Developmental Biology of Freshwater Fish [grant numbers 2020KF004].

语种：英文

外文关键词：Oryzias melastigma; Norethisterone; RNA-Seq; synthetic progestin; biomarkers

外文摘要：Norethisterone (NET) is one of the earliest synthetic progestins. Its widespread and long-term use means that NET is often detected on the surface and in groundwater samples, which may cause harm to aquatic organisms. Marine medaka (Oryzias melastigma) is a brackish fish, often used in ecotoxicology studies. In this study, the effect of norethisterone exposure on the transcriptome of marine medaka larvae was analyzed. Larvae were exposed to 10 ng/L NET for 24 h, then RNA-Seq was performed. Exposure to 10 ng/L NET resulted in 306 up-regulated and 516 down-regulated genes. An enrichment analysis revealed that differentially expressed genes (DEGs) were significantly enriched in metabolic and biosynthetic pathways, including DNA replication, proteasome, steroid biosynthesis, and other amino acid metabolism pathways. In the DNA replication pathway, all the DEGs were down-regulated, while in the steroid biosynthesis pathway, sqle, hmgcr, dhcr7, and dhcr24 were down-regulated. Quantitative real-time PCR (qPCR) was used to verify 12 DEGs, confirming that the validity of the RNA-Seq analysis. These results show that even low environmental concentrations of NET can lead to transcription level changes of genes related to fish growth and development, which may have adverse effects on the early development and growth of aquatic organisms.